Detection of pathogen based on the catalytic growth of gold nanocrystals

Xin Xing Li; Cuong Cao; Se Jong Han; Sang Jun Sim

doi:10.1016/j.watres.2008.12.024

Detection of pathogen based on the catalytic growth of gold nanocrystals

Xin Xing Li, Cuong Cao, Se Jong Han, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A homogenous detection of pathogen (Giardia lamblia cysts) based on the catalytic growth of gold nanoparticles (AuNPs) has been studied. In this study, centrifugal filters were employed as tools to concentrate and separate the pathogen cells, and moreover amplify the detection signal. The catalytic growth of gold nanoparticles was verified to be positively related to gold seeds concentration. On this basis, homogenous detection of the pathogenic bacteria in liquid phase was established by means of conjugating antibody to gold seeds. Under the given experimental condition, detection limit of G. lamblia cysts was determined as low as 1.088 × 10³ cells ml^-1. The additional nonspecific binding tests were also conducted to verify the detection specificity. This sensing platform has been proved to be a sensitive, reliable and simple method for large-scale pathogen detection, and provide valuable insight for the development of gold nanocrystals based colorimetric biosensors.

Original language	English
Pages (from-to)	1425-1431
Number of pages	7
Journal	Water Research
Volume	43
Issue number	5
DOIs	https://doi.org/10.1016/j.watres.2008.12.024
Publication status	Published - 2009 Mar
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) and Core Environmental Technology Development Project for Next Generation funded by the Ministry of Environment of the Republic of Korea.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Catalytic growth
Giardia lamblia cysts
Gold nanoparticles

ASJC Scopus subject areas

Ecological Modelling
Water Science and Technology
Waste Management and Disposal
Pollution

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10.1016/j.watres.2008.12.024

Cite this

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title = "Detection of pathogen based on the catalytic growth of gold nanocrystals",

abstract = "A homogenous detection of pathogen (Giardia lamblia cysts) based on the catalytic growth of gold nanoparticles (AuNPs) has been studied. In this study, centrifugal filters were employed as tools to concentrate and separate the pathogen cells, and moreover amplify the detection signal. The catalytic growth of gold nanoparticles was verified to be positively related to gold seeds concentration. On this basis, homogenous detection of the pathogenic bacteria in liquid phase was established by means of conjugating antibody to gold seeds. Under the given experimental condition, detection limit of G. lamblia cysts was determined as low as 1.088 × 103 cells ml-1. The additional nonspecific binding tests were also conducted to verify the detection specificity. This sensing platform has been proved to be a sensitive, reliable and simple method for large-scale pathogen detection, and provide valuable insight for the development of gold nanocrystals based colorimetric biosensors.",

keywords = "Catalytic growth, Giardia lamblia cysts, Gold nanoparticles",

author = "Li, {Xin Xing} and Cuong Cao and Han, {Se Jong} and Sim, {Sang Jun}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) and Core Environmental Technology Development Project for Next Generation funded by the Ministry of Environment of the Republic of Korea. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2009",

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doi = "10.1016/j.watres.2008.12.024",

language = "English",

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pages = "1425--1431",

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AU - Li, Xin Xing

AU - Cao, Cuong

AU - Han, Se Jong

AU - Sim, Sang Jun

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) and Core Environmental Technology Development Project for Next Generation funded by the Ministry of Environment of the Republic of Korea. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2009/3

Y1 - 2009/3

N2 - A homogenous detection of pathogen (Giardia lamblia cysts) based on the catalytic growth of gold nanoparticles (AuNPs) has been studied. In this study, centrifugal filters were employed as tools to concentrate and separate the pathogen cells, and moreover amplify the detection signal. The catalytic growth of gold nanoparticles was verified to be positively related to gold seeds concentration. On this basis, homogenous detection of the pathogenic bacteria in liquid phase was established by means of conjugating antibody to gold seeds. Under the given experimental condition, detection limit of G. lamblia cysts was determined as low as 1.088 × 103 cells ml-1. The additional nonspecific binding tests were also conducted to verify the detection specificity. This sensing platform has been proved to be a sensitive, reliable and simple method for large-scale pathogen detection, and provide valuable insight for the development of gold nanocrystals based colorimetric biosensors.

AB - A homogenous detection of pathogen (Giardia lamblia cysts) based on the catalytic growth of gold nanoparticles (AuNPs) has been studied. In this study, centrifugal filters were employed as tools to concentrate and separate the pathogen cells, and moreover amplify the detection signal. The catalytic growth of gold nanoparticles was verified to be positively related to gold seeds concentration. On this basis, homogenous detection of the pathogenic bacteria in liquid phase was established by means of conjugating antibody to gold seeds. Under the given experimental condition, detection limit of G. lamblia cysts was determined as low as 1.088 × 103 cells ml-1. The additional nonspecific binding tests were also conducted to verify the detection specificity. This sensing platform has been proved to be a sensitive, reliable and simple method for large-scale pathogen detection, and provide valuable insight for the development of gold nanocrystals based colorimetric biosensors.

KW - Catalytic growth

KW - Giardia lamblia cysts

KW - Gold nanoparticles

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Detection of pathogen based on the catalytic growth of gold nanocrystals

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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