Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)

Jun Hu Chen; Feng Lu; Chae Seung Lim; Jung Yeon Kim; Heui June Ahn; In Bum Suh; Satoru Takeo; Takafumi Tsuboi; Jetsumon Sattabongkot; Eun Taek Han

doi:10.1016/j.actatropica.2009.09.007

Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)

Jun Hu Chen, Feng Lu, Chae Seung Lim, Jung Yeon Kim, Heui June Ahn, In Bum Suh, Satoru Takeo, Takafumi Tsuboi, Jetsumon Sattabongkot, Eun Taek Han

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic.

Original language	English
Pages (from-to)	61-65
Number of pages	5
Journal	Acta Tropica
Volume	113
Issue number	1
DOIs	https://doi.org/10.1016/j.actatropica.2009.09.007
Publication status	Published - 2010 Jan

Bibliographical note

Funding Information:
This work was supported by the Korean Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) ( No. R01-2007-000-11260-0 ).

Keywords

Diagnosis
LAMP
Malaria
Plasmodium vivax
Republic of Korea

ASJC Scopus subject areas

Parasitology
veterinary (miscalleneous)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actatropica.2009.09.007

Cite this

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title = "Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)",

abstract = "Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic.",

keywords = "Diagnosis, LAMP, Malaria, Plasmodium vivax, Republic of Korea",

author = "Chen, {Jun Hu} and Feng Lu and Lim, {Chae Seung} and Kim, {Jung Yeon} and Ahn, {Heui June} and Suh, {In Bum} and Satoru Takeo and Takafumi Tsuboi and Jetsumon Sattabongkot and Han, {Eun Taek}",

note = "Funding Information: This work was supported by the Korean Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) ( No. R01-2007-000-11260-0 ).",

year = "2010",

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doi = "10.1016/j.actatropica.2009.09.007",

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AU - Chen, Jun Hu

AU - Lu, Feng

AU - Lim, Chae Seung

AU - Kim, Jung Yeon

AU - Ahn, Heui June

AU - Suh, In Bum

AU - Takeo, Satoru

AU - Tsuboi, Takafumi

AU - Sattabongkot, Jetsumon

AU - Han, Eun Taek

N1 - Funding Information: This work was supported by the Korean Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) ( No. R01-2007-000-11260-0 ).

PY - 2010/1

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N2 - Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic.

AB - Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic.

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Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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