Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample

P. Kristina Taylor; Sang Won Lee; Christophe Masselon; Ljiljana Pasa-Tolic; Deanna L. Auberry; Mary S. Lipton; Richard D. Smith

Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample

P. Kristina Taylor, Sang Won Lee, Christophe Masselon, Ljiljana Pasa-Tolic, Deanna L. Auberry, Mary S. Lipton, Richard D. Smith

Research output: Contribution to conference › Paper › peer-review

Abstract

Post-translational modifications were detected by comparison of intact proteins with tryptic digests in a complex biological sample. Bicinchoninic Acid (BCA) protein assay was utilized to determine protein concentration of samples for intact protein analysis. Final concentration was determined by BCA assay. The intact protein masses were examined to determine possible phosphorylated proteins that corresponded to proteins identified through enzymatic digestion. It was found that only a subset of proteins present in the sample was used to identify the intact protein masses, which reduces the number of mistaken identifications.

Original language	English
Pages	157-158
Number of pages	2
Publication status	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	02/6/2 → 02/6/6

ASJC Scopus subject areas

Spectroscopy

Cite this

Taylor, P. K., Lee, S. W., Masselon, C., Pasa-Tolic, L., Auberry, D. L., Lipton, M. S., & Smith, R. D. (2002). Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample. 157-158. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample. / Taylor, P. Kristina; Lee, Sang Won; Masselon, Christophe et al.
2002. 157-158 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Taylor, PK, Lee, SW, Masselon, C, Pasa-Tolic, L, Auberry, DL, Lipton, MS & Smith, RD 2002, 'Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 02/6/2 - 02/6/6 pp. 157-158.

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AU - Lipton, Mary S.

AU - Smith, Richard D.

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AB - Post-translational modifications were detected by comparison of intact proteins with tryptic digests in a complex biological sample. Bicinchoninic Acid (BCA) protein assay was utilized to determine protein concentration of samples for intact protein analysis. Final concentration was determined by BCA assay. The intact protein masses were examined to determine possible phosphorylated proteins that corresponded to proteins identified through enzymatic digestion. It was found that only a subset of proteins present in the sample was used to identify the intact protein masses, which reduces the number of mistaken identifications.

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ER -

Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample

Abstract

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ASJC Scopus subject areas

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