Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Jiho Min; Chang Woo Lee; Seung Hyeun Moon; Robert A. LaRossa; Man Bock Gu

doi:10.1007/PL00007683

Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Jiho Min, Chang Woo Lee, Seung Hyeun Moon, Robert A. LaRossa, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::IuxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein- damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma- rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.

Original language	English
Pages (from-to)	41-45
Number of pages	5
Journal	Radiation and Environmental Biophysics
Volume	39
Issue number	1
DOIs	https://doi.org/10.1007/PL00007683
Publication status	Published - 2000 Mar
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements We are grateful to Ms. Eun Jin Kim for her technical assistance in the preparation of samples. This work was supported by the Science and Technology Policy Institute (STEPI) in Korea (Grant no. 97-B-01-08) and supported in part by the Korea Science and Engineering Foundation (KOSEF) through ADEMRC at Kwangju Institute of Science and Technology (K-JIST).

Keywords

Bioluminescent bacteria
DNA damage
Ionizing radiation biosensor
Oxidative damage
Protein damage
Stress promoters

ASJC Scopus subject areas

Biophysics
Radiation
General Environmental Science

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10.1007/PL00007683

Cite this

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title = "Detection of radiation effects using recombinant bioluminescent Escherichia coli strains",

abstract = "Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::IuxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein- damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma- rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.",

keywords = "Bioluminescent bacteria, DNA damage, Ionizing radiation biosensor, Oxidative damage, Protein damage, Stress promoters",

author = "Jiho Min and Lee, {Chang Woo} and Moon, {Seung Hyeun} and LaRossa, {Robert A.} and Gu, {Man Bock}",

note = "Funding Information: Acknowledgements We are grateful to Ms. Eun Jin Kim for her technical assistance in the preparation of samples. This work was supported by the Science and Technology Policy Institute (STEPI) in Korea (Grant no. 97-B-01-08) and supported in part by the Korea Science and Engineering Foundation (KOSEF) through ADEMRC at Kwangju Institute of Science and Technology (K-JIST).",

year = "2000",

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doi = "10.1007/PL00007683",

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AU - Min, Jiho

AU - Lee, Chang Woo

AU - Moon, Seung Hyeun

AU - LaRossa, Robert A.

AU - Gu, Man Bock

N1 - Funding Information: Acknowledgements We are grateful to Ms. Eun Jin Kim for her technical assistance in the preparation of samples. This work was supported by the Science and Technology Policy Institute (STEPI) in Korea (Grant no. 97-B-01-08) and supported in part by the Korea Science and Engineering Foundation (KOSEF) through ADEMRC at Kwangju Institute of Science and Technology (K-JIST).

PY - 2000/3

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N2 - Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::IuxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein- damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma- rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.

AB - Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::IuxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein- damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma- rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.

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Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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