Abstract
A simple method was developed for the analysis of seven stilbene-type fluorescent whitening agents (FWAs) in paper materials by ion-pair reversed-phase high-performance liquid chromatography with fluorescence detection. These stilbene-type FWAs included two disulfonate, two tetrasulfonate, and three hexasulfonate compounds. After optimization of chromatographic conditions, the FWAs were satisfactorily separated using a reversed-phase column (RP-18) with the following isocratic mobile phase: methanol-water (60:40) containing 17.5 mM TBABr and 10 mM citrate buffer (pH = 7.0). The calibration plot was linear in the range from 5 to 500 ng/mL for two disulfo-FWAs and from 1 to 500 ng/mL for the other five FWAs. Precision levels of the calibration curve as indicated by RSD of response factors were 1.2 and 8.1%. Limits of quantitation (LOQ) ranged from 1.2 to 11 ng/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 3971-3976 |
| Number of pages | 6 |
| Journal | Bulletin of the Korean Chemical Society |
| Volume | 33 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2012 Dec 20 |
Keywords
- Fluorescent whitening agents
- Ion-pair chromatography
- Paper materials
ASJC Scopus subject areas
- Chemistry(all)