Abstract
A highly sensitive analytical tool for the fast quantification of irsogladine in human plasma was developed. Cleanup using a solid-phase extraction technique is a simple method for extracting both irsogladine and lamotrigine (internal standard) spiked into human plasma: 89.4 ± 2.4% for irsogladine and 85.9 ± 3.4% for lamotrigine. The resolvable separation of both analytes through reversed-phase high-performance liquid chromatography (HPLC) was carried out within 5 min. The HPLC-electrospray ionization (ESI)-tandem mass spectrometry (MS) method, which was operated in a selected reaction monitoring mode specific to the target analytes, was verified for use in the quantification of irsogladine. The inter- and intra-day precision (RSD) were <4% and their accuracies were between 85.9 to 89.8%. The calibration curve for irsogladine spiked into human plasma was linear over the range from 1 to 100 ng/mL; the limit of quantification was estimated to be 1.8 ng/mL. The established method was successfully applied for a bioequivalence study of irsogladine.
| Original language | English |
|---|---|
| Pages (from-to) | 1894-1898 |
| Number of pages | 5 |
| Journal | Latin American Journal of Pharmacy |
| Volume | 35 |
| Issue number | 8 |
| Publication status | Published - 2016 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea (NRF) grant (2015R1A2A2A01002524), and by the grant (PJ011066) funded by the Next-Generation BioGreen21 program, Rural Development Administration.
Publisher Copyright:
© 2016, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Keywords
- Bioequivalence study
- HPLC-ESI-MS/MS
- Human plasma
- Irsogladine
ASJC Scopus subject areas
- Pharmaceutical Science