Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)

Hyunjung Lim; Seung Min Back; Jeonghun Nam; Hyuk Choi

doi:10.1371/journal.pone.0197619

Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)

Hyunjung Lim
, Seung Min Back
, Jeonghun Nam^*
, Hyuk Choi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CI_normal-CI_hardened = 0.195).

Original language	English
Article number	e0197619
Journal	PloS one
Volume	13
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0197619
Publication status	Published - 2018 May

Bibliographical note

Publisher Copyright:
© 2018 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ASJC Scopus subject areas

General

Access to Document

10.1371/journal.pone.0197619

Cite this

@article{46dec344cca44ba08bf74af44f70f175,

title = "Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)",

abstract = "Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CInormal-CIhardened = 0.195).",

author = "Hyunjung Lim and Back, \{Seung Min\} and Jeonghun Nam and Hyuk Choi",

note = "Publisher Copyright: {\textcopyright} 2018 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2018",

month = may,

doi = "10.1371/journal.pone.0197619",

language = "English",

volume = "13",

journal = "PloS one",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "5",

}

TY - JOUR

T1 - Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)

AU - Lim, Hyunjung

AU - Back, Seung Min

AU - Nam, Jeonghun

AU - Choi, Hyuk

N1 - Publisher Copyright: © 2018 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2018/5

Y1 - 2018/5

N2 - Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CInormal-CIhardened = 0.195).

AB - Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CInormal-CIhardened = 0.195).

UR - https://www.scopus.com/pages/publications/85047447727

U2 - 10.1371/journal.pone.0197619

DO - 10.1371/journal.pone.0197619

M3 - Article

C2 - 29787582

AN - SCOPUS:85047447727

SN - 1932-6203

VL - 13

JO - PloS one

JF - PloS one

IS - 5

M1 - e0197619

ER -

Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this