Abstract
Ribosomal protein genes (RPG), which are scattered throughout the genomes of all eukaryotes, are subjected to coordinated expression. In yeast, the expression of RPGs is highly regulated, mainly at the transcriptional level. Recent research has found that many ribosomal proteins (RPs) function in multiple processes in addition to protein synthesis. Therefore, detailed knowledge of promoter architecture as well as gene regulation is important in understanding the multiple cellular processes mediated by RPGs. In this study, we investigated the functional architecture of the yeast RPS3 promoter and identified many putative cis-elements. Using β-galactosidase reporter analysis and EMSA, the core promoter of RPS3 containing UAS rpg and T-rich regions was corroborated. Moreover, the promoter occupancy of RPS3 by three transcription factors was confirmed. Taken together, our results further the current understanding of the promoter architecture and trans-elements of the Saccharomyces cerevisiae RPS3 gene.
| Original language | English |
|---|---|
| Pages (from-to) | 741-750 |
| Number of pages | 10 |
| Journal | Biochimica et Biophysica Acta - Gene Regulatory Mechanisms |
| Volume | 1789 |
| Issue number | 11-12 |
| DOIs | |
| Publication status | Published - 2009 Nov |
Bibliographical note
Funding Information:This work was supported in part by Proteomics grants FPR05C2-390 and KRF-2008-C00787 .
Keywords
- Rap1p
- Ribosomal protein gene
- T-rich region
- Transcription factor
- UAS
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics