Determination of tocopherol contents in refined edible oils using an HPLC method

Jiang Ning Hu; Xue Mei Zhu; Prakash Adhikari; Dan Li; In Hwan Kim; Ki Teak Lee

doi:10.3746/jfn.2009.14.3.260

Determination of tocopherol contents in refined edible oils using an HPLC method

Jiang Ning Hu, Xue Mei Zhu, Prakash Adhikari, Dan Li, In Hwan Kim, Ki Teak Lee

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r ²>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.

Original language	English
Pages (from-to)	260-264
Number of pages	5
Journal	Journal of Food Science and Nutrition
Volume	14
Issue number	3
DOIs	https://doi.org/10.3746/jfn.2009.14.3.260
Publication status	Published - 2009

Keywords

HPLC separation
Refined edible oils
Tocopherols

ASJC Scopus subject areas

Food Science
Nutrition and Dietetics

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10.3746/jfn.2009.14.3.260

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title = "Determination of tocopherol contents in refined edible oils using an HPLC method",

abstract = "A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r 2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.",

keywords = "HPLC separation, Refined edible oils, Tocopherols",

author = "Hu, {Jiang Ning} and Zhu, {Xue Mei} and Prakash Adhikari and Dan Li and Kim, {In Hwan} and Lee, {Ki Teak}",

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AU - Hu, Jiang Ning

AU - Zhu, Xue Mei

AU - Adhikari, Prakash

AU - Li, Dan

AU - Kim, In Hwan

AU - Lee, Ki Teak

PY - 2009

Y1 - 2009

N2 - A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r 2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.

AB - A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r 2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.

KW - HPLC separation

KW - Refined edible oils

KW - Tocopherols

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JO - Preventive Nutrition and Food Science

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Determination of tocopherol contents in refined edible oils using an HPLC method

Abstract

Keywords

ASJC Scopus subject areas

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