Development of a bifunctional BODIPY probe for mitochondria imaging and in situ photo-crosslinking in live cell

Dhiraj P. Murale, Md Mamunul Haque, Seong Cheol Hong, Se young Jang, Jung Hoon Lee, Seo Jeong An, Jun Seok Lee

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Mitochondria are eukaryotic sub-cellular organelle that play critical roles in energy generation for life. As the morphology of the mitochondria is dramatically versatile, there is a great demand for easy-to-use tools for mitochondria imaging. Until today, many organic probes are known to target mitochondria in live-cell conditions, but many of them are suffered from continuous leaking out from mitochondria. To overcome this challenge, we developed a bifunctional BODIPY probe that selectively engaged mitochondria and spontaneously produced the chemical crosslinking by photo-activation. To systematically optimize the dual functions, we synthesized five BODIPY fluorophores and investigated their photophysical and photo-crosslinking properties by comparing series of photo-crosslinking functional groups and their modification positions in BODIPY fluorophore. Finally, we discovered not only pcBDaza-Mito selectively stains mitochondria in live-cell condition, but also it exhibited 2.3 folds increase for photo-crosslinking efficiency to mitochondria proteins. These results present that the bifunctional BODIPY probe has great merit for robust mitochondria imaging in a live cell.

Original languageEnglish
Article number109830
JournalDyes and Pigments
Volume196
DOIs
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
This work is supported by a Korea University Grant ( K2110571 ), the National Research Foundation ( NRF-2017M3A9D8029942 , NRF‐2018M3A9H4079286 , NRF‐2020R1A2C2004422 ), and KIST intramural funding ( 2E31093 ). Dr. Haque is a recipient of the Brain Pool Program through NRF (NRF-2018H1D3A1A02074556).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • Bifunctional probe
  • Fluorescent probe
  • Live cell sub-organelle imaging
  • Mitochondria
  • Photo-crosslinking probe

ASJC Scopus subject areas

  • General Chemical Engineering
  • Process Chemistry and Technology

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