Mitochondria are eukaryotic sub-cellular organelle that play critical roles in energy generation for life. As the morphology of the mitochondria is dramatically versatile, there is a great demand for easy-to-use tools for mitochondria imaging. Until today, many organic probes are known to target mitochondria in live-cell conditions, but many of them are suffered from continuous leaking out from mitochondria. To overcome this challenge, we developed a bifunctional BODIPY probe that selectively engaged mitochondria and spontaneously produced the chemical crosslinking by photo-activation. To systematically optimize the dual functions, we synthesized five BODIPY fluorophores and investigated their photophysical and photo-crosslinking properties by comparing series of photo-crosslinking functional groups and their modification positions in BODIPY fluorophore. Finally, we discovered not only pcBDaza-Mito selectively stains mitochondria in live-cell condition, but also it exhibited 2.3 folds increase for photo-crosslinking efficiency to mitochondria proteins. These results present that the bifunctional BODIPY probe has great merit for robust mitochondria imaging in a live cell.
Bibliographical noteFunding Information:
This work is supported by a Korea University Grant ( K2110571 ), the National Research Foundation ( NRF-2017M3A9D8029942 , NRF‐2018M3A9H4079286 , NRF‐2020R1A2C2004422 ), and KIST intramural funding ( 2E31093 ). Dr. Haque is a recipient of the Brain Pool Program through NRF (NRF-2018H1D3A1A02074556).
© 2021 Elsevier Ltd
- Bifunctional probe
- Fluorescent probe
- Live cell sub-organelle imaging
- Photo-crosslinking probe
ASJC Scopus subject areas
- Chemical Engineering(all)
- Process Chemistry and Technology