Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes

Honggu Chun, Patty J. Dennis, Erin R. Ferguson Welch, Jean Pierre Alarie, James W. Jorgenson, J. Michael Ramsey

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.

    Original languageEnglish
    Pages (from-to)140-147
    Number of pages8
    JournalJournal of Chromatography A
    Volume1523
    DOIs
    Publication statusPublished - 2017 Nov 10

    Bibliographical note

    Publisher Copyright:
    © 2017 Elsevier B.V.

    Keywords

    • Label-free detection
    • Microfluidics
    • Photothermal absorbance detection
    • Polyelectrolytic gel electrode

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Organic Chemistry

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