TY - JOUR
T1 - Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes
AU - Chun, Honggu
AU - Dennis, Patty J.
AU - Ferguson Welch, Erin R.
AU - Alarie, Jean Pierre
AU - Jorgenson, James W.
AU - Ramsey, J. Michael
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11/10
Y1 - 2017/11/10
N2 - The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.
AB - The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.
KW - Label-free detection
KW - Microfluidics
KW - Photothermal absorbance detection
KW - Polyelectrolytic gel electrode
UR - http://www.scopus.com/inward/record.url?scp=85021273989&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2017.06.053
DO - 10.1016/j.chroma.2017.06.053
M3 - Article
C2 - 28668370
AN - SCOPUS:85021273989
SN - 0021-9673
VL - 1523
SP - 140
EP - 147
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -