Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications

Yuna Jung; Ji Hye Jin; Youngseo Kim; Ji Hyeon Oh; Heechang Moon; Huisu Jeong; Jaehoon Kim; Yoon Kyung Park; Yohan Oh; Sungnam Park; Dokyoung Kim

doi:10.1039/d2ob00830k

Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications

Yuna Jung, Ji Hye Jin, Youngseo Kim, Ji Hyeon Oh, Heechang Moon, Huisu Jeong, Jaehoon Kim, Yoon Kyung Park, Yohan Oh, Sungnam Park, Dokyoung Kim

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH₂) and hydrophobic (-C₁₂H₂₅) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.

Original language	English
Pages (from-to)	5423-5433
Number of pages	11
Journal	Organic and Biomolecular Chemistry
Volume	20
Issue number	27
DOIs	https://doi.org/10.1039/d2ob00830k
Publication status	Published - 2022 Jun 21

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (2018-R1A6A1A03025124; D. K.). This research was also supported by the Bio & Medical Technology Development Program of the NRF of Korea funded by the Ministry of Science & ICT (2022-R1F1A1069954, 2022-M3A9H1014157, 2021-M3A9I5030523; D. K.) and a grant from the Korea Health Technology R&D Project of the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI21C0239; D. K.).

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/d2ob00830k

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title = "Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications",

abstract = "Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH2) and hydrophobic (-C12H25) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.",

author = "Yuna Jung and Jin, {Ji Hye} and Youngseo Kim and Oh, {Ji Hyeon} and Heechang Moon and Huisu Jeong and Jaehoon Kim and Park, {Yoon Kyung} and Yohan Oh and Sungnam Park and Dokyoung Kim",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (2018-R1A6A1A03025124; D. K.). This research was also supported by the Bio & Medical Technology Development Program of the NRF of Korea funded by the Ministry of Science & ICT (2022-R1F1A1069954, 2022-M3A9H1014157, 2021-M3A9I5030523; D. K.) and a grant from the Korea Health Technology R&D Project of the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI21C0239; D. K.). Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry.",

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doi = "10.1039/d2ob00830k",

language = "English",

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T1 - Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications

AU - Jung, Yuna

AU - Jin, Ji Hye

AU - Kim, Youngseo

AU - Oh, Ji Hyeon

AU - Moon, Heechang

AU - Jeong, Huisu

AU - Kim, Jaehoon

AU - Park, Yoon Kyung

AU - Oh, Yohan

AU - Park, Sungnam

AU - Kim, Dokyoung

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education (2018-R1A6A1A03025124; D. K.). This research was also supported by the Bio & Medical Technology Development Program of the NRF of Korea funded by the Ministry of Science & ICT (2022-R1F1A1069954, 2022-M3A9H1014157, 2021-M3A9I5030523; D. K.) and a grant from the Korea Health Technology R&D Project of the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI21C0239; D. K.). Publisher Copyright: © 2022 The Royal Society of Chemistry.

PY - 2022/6/21

Y1 - 2022/6/21

N2 - Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH2) and hydrophobic (-C12H25) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.

AB - Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH2) and hydrophobic (-C12H25) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.

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U2 - 10.1039/d2ob00830k

DO - 10.1039/d2ob00830k

M3 - Article

C2 - 35758412

AN - SCOPUS:85133211420

SN - 1477-0520

VL - 20

SP - 5423

EP - 5433

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 27

ER -

Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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