Development of a novel cell surface attachment system to display multi-protein complex using the cohesin-dockerin binding pair

Hyeok Jin Ko, Heesang Song, In Geol Choi

Research output: Contribution to journalArticlepeer-review

Abstract

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a non-covalent protein–protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from Archaeoglobus fulgidus to build the CSA system. Then, we validated the orthogonal Coh-Doc binding by attaching a monomeric red fluorescent protein to the cell surface. In addition, we evaluated the functional anchoring of proteins fused with the Doc module to the autodisplayed Coh module on the surface of Escherichia coli. The designed CSA system was applied to create a functional attachment of dimeric α-neoagarobiose hydrolase to the surface of E. coli cells.

Original languageEnglish
Pages (from-to)1183-1189
Number of pages7
JournalJournal of microbiology and biotechnology
Volume31
Issue number8
DOIs
Publication statusPublished - 2021 Aug 28

Bibliographical note

Funding Information:
This study was supported by a grant from Chosun University (2021).

Publisher Copyright:
Copyright © 2021 by The Korean Society for Microbiology and Biotechnology.

Keywords

  • Cohesin–dockerin
  • Escherichia coli cell surface attachment
  • Non-covalent interaction module
  • α-neoagarobiose hydrolase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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