Development of an effective sample preparation method for the proteome analysis of body fluids using 2-d gel electrophoresis

Won A. Joo; Do Youn Lee; Chan Wha Kim

doi:10.1271/bbb.67.1574

Development of an effective sample preparation method for the proteome analysis of body fluids using 2-d gel electrophoresis

Won A. Joo, Do Youn Lee, Chan Wha Kim

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.

Original language	English
Pages (from-to)	1574-1577
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	7
DOIs	https://doi.org/10.1271/bbb.67.1574
Publication status	Published - 2003 Jan 1

Keywords

Body fluids
Centrifugal filter device, detergent
IEF
Sample preparation of 2-DE

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.67.1574

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abstract = "A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.",

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Development of an effective sample preparation method for the proteome analysis of body fluids using 2-d gel electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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