Development of an experimental model for ocular toxicity screening in Zebrafish

Soonil Koun; Youngsub Eom; Mi Jeong Kim; Suhyun Kim; Ik Hyun Lee; Hae Chul Park; Jong-Suk Song; Hyo Myung Kim

doi:10.1016/j.bbrc.2021.04.102

Development of an experimental model for ocular toxicity screening in Zebrafish

Soonil Koun, Youngsub Eom, Mi Jeong Kim, Suhyun Kim, Ik Hyun Lee, Hae Chul Park, Jong-Suk Song, Hyo Myung Kim

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Background: To investigate the efficacy of a novel experimental model for exploring visual function using a contrast-optomotor response (C-OMR) assay made by applying the contrast sensitivity test to the OMR assay in zebrafish. Methods: Zebrafish larvae were treated with 0 (control), 5, 10, or 15 μM gentamicin and digoxin for 24 h at four days post-fertilization (dpf). Zebrafish larvae were assessed using the C-OMR assay with graded contrast gray-white stripes at 5 dpf, and the results were expressed as the percentage of larvae that finished swimming for 30 s (n = 20 per each group). The same C-OMR assay was repeated four times using different larvae. Results: The percentage of larvae that finished swimming within 30 s was significantly reduced in larvae treated with 5, 10, and 15 μM gentamicin and 10 and 15 μM digoxin as compared to the Control groups. The C-OMR assay could distinguish that the decrease in visual function was different depending on the concentration of gentamicin and digoxin (5, 10, and 15 μM), whereas the OMR test with one contrast gray-white stripe could not. Conclusions: The method of analyzing zebrafish OMR using graded contrast gray-white stripes is more sensitive than the OMR assay alone and may be more useful for assessing the drug toxicity and eye-related diseases to improve the understanding of drug-induced ocular side effects in the clinic.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	559
DOIs	https://doi.org/10.1016/j.bbrc.2021.04.102
Publication status	Published - 2021 Jun 25

Bibliographical note

Funding Information:
This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: 9991007583, KMDF_PR_20200901_0296); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2018R1C1B6002794); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) (2020002960007, NTIS-1485017184); and by Priority Research Centers Program (2017R1A6A1A03015562) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education. There are patents resulting from the work reported in this manuscript (PCT/KR2019/010224).

Funding Information:
This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety ) (Project Number: 9991007583 , KMDF_PR_20200901_0296 ); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2018R1C1B6002794 ); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) ( 2020002960007 , NTIS-1485017184 ); and by Priority Research Centers Program ( 2017R1A6A1A03015562 ) through the National Research Foundation of Korea ( NRF ) funded by the Ministry of Education .

Publisher Copyright:
© 2021 The Authors

Keywords

Experimental model
Ocular toxicity
Visual function
Zebrafish

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2021.04.102

Cite this

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title = "Development of an experimental model for ocular toxicity screening in Zebrafish",

abstract = "Background: To investigate the efficacy of a novel experimental model for exploring visual function using a contrast-optomotor response (C-OMR) assay made by applying the contrast sensitivity test to the OMR assay in zebrafish. Methods: Zebrafish larvae were treated with 0 (control), 5, 10, or 15 μM gentamicin and digoxin for 24 h at four days post-fertilization (dpf). Zebrafish larvae were assessed using the C-OMR assay with graded contrast gray-white stripes at 5 dpf, and the results were expressed as the percentage of larvae that finished swimming for 30 s (n = 20 per each group). The same C-OMR assay was repeated four times using different larvae. Results: The percentage of larvae that finished swimming within 30 s was significantly reduced in larvae treated with 5, 10, and 15 μM gentamicin and 10 and 15 μM digoxin as compared to the Control groups. The C-OMR assay could distinguish that the decrease in visual function was different depending on the concentration of gentamicin and digoxin (5, 10, and 15 μM), whereas the OMR test with one contrast gray-white stripe could not. Conclusions: The method of analyzing zebrafish OMR using graded contrast gray-white stripes is more sensitive than the OMR assay alone and may be more useful for assessing the drug toxicity and eye-related diseases to improve the understanding of drug-induced ocular side effects in the clinic.",

keywords = "Experimental model, Ocular toxicity, Visual function, Zebrafish",

author = "Soonil Koun and Youngsub Eom and Kim, {Mi Jeong} and Suhyun Kim and Lee, {Ik Hyun} and Park, {Hae Chul} and Jong-Suk Song and Kim, {Hyo Myung}",

note = "Funding Information: This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: 9991007583, KMDF_PR_20200901_0296); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2018R1C1B6002794); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) (2020002960007, NTIS-1485017184); and by Priority Research Centers Program (2017R1A6A1A03015562) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education. There are patents resulting from the work reported in this manuscript (PCT/KR2019/010224). Funding Information: This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety ) (Project Number: 9991007583 , KMDF_PR_20200901_0296 ); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2018R1C1B6002794 ); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) ( 2020002960007 , NTIS-1485017184 ); and by Priority Research Centers Program ( 2017R1A6A1A03015562 ) through the National Research Foundation of Korea ( NRF ) funded by the Ministry of Education . Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = jun,

day = "25",

doi = "10.1016/j.bbrc.2021.04.102",

language = "English",

volume = "559",

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T1 - Development of an experimental model for ocular toxicity screening in Zebrafish

AU - Koun, Soonil

AU - Eom, Youngsub

AU - Kim, Mi Jeong

AU - Kim, Suhyun

AU - Lee, Ik Hyun

AU - Park, Hae Chul

AU - Song, Jong-Suk

AU - Kim, Hyo Myung

N1 - Funding Information: This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: 9991007583, KMDF_PR_20200901_0296); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2018R1C1B6002794); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) (2020002960007, NTIS-1485017184); and by Priority Research Centers Program (2017R1A6A1A03015562) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education. There are patents resulting from the work reported in this manuscript (PCT/KR2019/010224). Funding Information: This paper was supported by the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety ) (Project Number: 9991007583 , KMDF_PR_20200901_0296 ); and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( 2018R1C1B6002794 ); by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) ( 2020002960007 , NTIS-1485017184 ); and by Priority Research Centers Program ( 2017R1A6A1A03015562 ) through the National Research Foundation of Korea ( NRF ) funded by the Ministry of Education . Publisher Copyright: © 2021 The Authors

PY - 2021/6/25

Y1 - 2021/6/25

N2 - Background: To investigate the efficacy of a novel experimental model for exploring visual function using a contrast-optomotor response (C-OMR) assay made by applying the contrast sensitivity test to the OMR assay in zebrafish. Methods: Zebrafish larvae were treated with 0 (control), 5, 10, or 15 μM gentamicin and digoxin for 24 h at four days post-fertilization (dpf). Zebrafish larvae were assessed using the C-OMR assay with graded contrast gray-white stripes at 5 dpf, and the results were expressed as the percentage of larvae that finished swimming for 30 s (n = 20 per each group). The same C-OMR assay was repeated four times using different larvae. Results: The percentage of larvae that finished swimming within 30 s was significantly reduced in larvae treated with 5, 10, and 15 μM gentamicin and 10 and 15 μM digoxin as compared to the Control groups. The C-OMR assay could distinguish that the decrease in visual function was different depending on the concentration of gentamicin and digoxin (5, 10, and 15 μM), whereas the OMR test with one contrast gray-white stripe could not. Conclusions: The method of analyzing zebrafish OMR using graded contrast gray-white stripes is more sensitive than the OMR assay alone and may be more useful for assessing the drug toxicity and eye-related diseases to improve the understanding of drug-induced ocular side effects in the clinic.

AB - Background: To investigate the efficacy of a novel experimental model for exploring visual function using a contrast-optomotor response (C-OMR) assay made by applying the contrast sensitivity test to the OMR assay in zebrafish. Methods: Zebrafish larvae were treated with 0 (control), 5, 10, or 15 μM gentamicin and digoxin for 24 h at four days post-fertilization (dpf). Zebrafish larvae were assessed using the C-OMR assay with graded contrast gray-white stripes at 5 dpf, and the results were expressed as the percentage of larvae that finished swimming for 30 s (n = 20 per each group). The same C-OMR assay was repeated four times using different larvae. Results: The percentage of larvae that finished swimming within 30 s was significantly reduced in larvae treated with 5, 10, and 15 μM gentamicin and 10 and 15 μM digoxin as compared to the Control groups. The C-OMR assay could distinguish that the decrease in visual function was different depending on the concentration of gentamicin and digoxin (5, 10, and 15 μM), whereas the OMR test with one contrast gray-white stripe could not. Conclusions: The method of analyzing zebrafish OMR using graded contrast gray-white stripes is more sensitive than the OMR assay alone and may be more useful for assessing the drug toxicity and eye-related diseases to improve the understanding of drug-induced ocular side effects in the clinic.

KW - Experimental model

KW - Ocular toxicity

KW - Visual function

KW - Zebrafish

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DO - 10.1016/j.bbrc.2021.04.102

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VL - 559

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JO - Biochemical and biophysical research communications

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ER -

Development of an experimental model for ocular toxicity screening in Zebrafish

Abstract

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Keywords

ASJC Scopus subject areas

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