With the advances in organoid culture, patient-derived organoids are utilized in diverse fields to broaden our understanding of conventional 2-dimensional (2D) culture methods and animal models. Patient-derived organoids have found new applications, such as screening for patient-matching drugs, immune checkpoint drugs, and mutation-target drugs, in the field of drug screening. However, conventional dome-shaped Matrigel drop-based screening methods using 24-, 48-, and 96-well plates are not effective for carrying out large-scale drug screening using organoids. Here, we present a newly developed 96-well plate-based method for the effective screening of patient-derived tumor organoids embedded in Matrigel. The new screening plate has a central hole with a diameter of 3 or 5 mm to provide a definite space for placing Matrigel in a cylindrical shape. Compared to the conventional dome-shaped Matrigel where the Matrigel drop is located arbitrarily, a cylinder-shaped Matrigel position in confined central wells allowed for faster and cost-effective tumor organoid screening. Importantly, the cylinder-shaped Matrigel ensures better consistency in high-throughput image-based analysis, which is used worldwide. Our results demonstrate the possibility of replacing the conventional 24-, 48-, and 96-well plates with the newly developed plates for effective tumor organoid screening.
Bibliographical noteFunding Information:
This work was supported by the Technology Innovation Program (No.20012378), funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea). This work was supported by the Technology Innovation Program (No.20008413), funded by the Korean government (MSIT). This work was supported by the Agency for Defense Development, Republic of Korea (UE211118ZD, 311KK5-912888601).
© 2023, The Author(s).
- Cancer organoid
- Drug screening
- Organoid platform
ASJC Scopus subject areas
- Biomedical Engineering
- Electrical and Electronic Engineering