Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol

Laxmi Prasad Thapa; Sang Jun Lee; Hah Young Yoo; Han Suk Choi; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.enzmictec.2013.04.009

Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol

Laxmi Prasad Thapa, Sang Jun Lee, Hah Young Yoo, Han Suk Choi, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.

Original language	English
Pages (from-to)	206-215
Number of pages	10
Journal	Enzyme and Microbial Technology
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/j.enzmictec.2013.04.009
Publication status	Published - 2013 Aug 15

Keywords

Bioethanol production
Enterobacter aerogenes
Genetic manipulation
Recombinant strains

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2013.04.009

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title = "Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol",

abstract = "production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.",

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AU - Lee, Sang Jun

AU - Yoo, Hah Young

AU - Choi, Han Suk

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2013/8/15

Y1 - 2013/8/15

N2 - production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.

AB - production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.

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KW - Recombinant strains

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Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol

Abstract

Keywords

ASJC Scopus subject areas

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