Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol

Laxmi Prasad Thapa, Sang Jun Lee, Hah Young Yoo, Han Suk Choi, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers.

Original languageEnglish
Pages (from-to)206-215
Number of pages10
JournalEnzyme and Microbial Technology
Issue number3
Publication statusPublished - 2013 Aug 15


  • Bioethanol production
  • Enterobacter aerogenes
  • Genetic manipulation
  • Recombinant strains

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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