Abstract
Electrochemical nano‐biosensor systems are popular in the industrial field, along with evaluations of medical, agricultural, environmental and sports analysis, because they can simultaneously perform qualitative and quantitative analyses with high sensitivity. However, real‐time detection using an electrochemical nano‐biosensor is greatly affected by the surrounding environment with the performance of the electron transport materials. Therefore, many researchers are trying to find good factors for real‐time detection. In this work, it was found that a composite composed of graphite oxide/cobalt/chitosan had strong stability and electron transfer capability and was applied to a bioelectrochemical nano‐biosensor with high sensitivity and stability. As a mediator‐modified electrode, the GO/Co/chitosan composite was electrically deposited onto an Au film electrode by covalent boding, while glucose oxidase as a receptor was immobilized on the end of the GO/Co/chitosan composite. It was confirmed that the electron transfer ability of the GO/Co/chitosan composite was excellent, as shown with power density analysis. In addition, the real‐time detection of D‐glucose could be successfully performed by the developed nano‐biosensor with a high range of detected concentrations from 1.0 to 15.0 mM. Furthermore, the slope value composed of the current, per the concentration of D‐glucose as a detection response, was significantly maintained even after 14 days.
Original language | English |
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Article number | 464 |
Journal | Biosensors |
Volume | 12 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2022 Jul |
Bibliographical note
Funding Information:This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (MSIP; no. 2014R1A2A2A01007323, no. 2019R1A2C1006793, no. 2021R1F1A1055676)) and the Industrial Strate-gic Technology Development Program (10051513) funded by the Ministry of Trade, Industry and Energy (MI, Korea).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
- D‐glucose
- GO/Co/chitosan
- glucose oxidase
- nano‐biosensor
- real‐time detection
ASJC Scopus subject areas
- Analytical Chemistry
- Biotechnology
- Biomedical Engineering
- Instrumentation
- Engineering (miscellaneous)
- Clinical Biochemistry