Abstract
A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in situ removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis membrane and equipped on the agitator of spinner reactor for in situ removal of ammonium ion. The semi-fed-batch operation was employed to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.
| Original language | English |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2000 |
Bibliographical note
Funding Information:Acknowledgements This study was supported by
Funding Information:
Biochemical Engineering Research Grant from the Ministry of Education, Korea in 1996.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- CHO cells
- Microcarrier
- Semi-fed-batch
- Zeolite
- in situ ammonium ion removal
- tPA
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering