Abstract
This paper describes a multi-well-based (24-well) electrochemical dissolved oxygen sensor (m-well ECDO sensor) array on a glass substrate, using Nafion as a solid polymer electrolyte. The designed m- well ECDO sensor array shares working electrodes, reference electrodes, and counter electrodes, to reduce the number of electrode pads to approximately one-fourth of its expected pad numbers (72 to 18), compared to the simply repeated 24 sensors. This m-well ECDO sensor array can simultaneously measure up to six sensors at individual crossing points of the row and column electrodes. The fabricated m-well ECDO sensor array showed good repeatability during five consecutive measurements at intervals of 100 s with a 2.4 μm thick Nafion membrane. The response time was about 10 s when the solution state changed from the air-saturated state to the zero-oxygen state, and the measured output current was about -1 μA and-20 nA in the corresponding states, respectively. The fabricated m-well ECDO sensor array was applied to measure the oxygen consumption rate of C2C12 cells for a cell metabolic study. The ratios of the uncoupled oxygen consumption rate (OCR) to the coupled OCR obtained from three sensors of the fabricated m-well ECDO sensor array were 2.16, 2.22, 2.18, which are higher than 1.71, 1.68, 1.73 obtained from of the XF-24 of Agilent Inc. in 5,000, 10,000, 30,000 cells, respectively. This indicates that using the fabricated m-well ECDO sensor array, variations in DO (dissolved oxygen) concentration due to cellular respiration can be effectively detected, even at low cell numbers.
Original language | English |
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Article number | 127465 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 306 |
DOIs | |
Publication status | Published - 2020 Mar 1 |
Bibliographical note
Funding Information:This work was supported by the R&D program of MOTIE/KEIT. [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells]
Funding Information:
This work was supported by the R&D program of MOTIE/KEIT . [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells] Appendix A
Publisher Copyright:
© 2019 Elsevier B.V.
Keywords
- Cell metabolism
- Dissolved oxygen sensor
- Electrochemical sensor
- Multi-well based sensor array
- Oxygen consumption rate
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry