Development of multi-well-based electrochemical dissolved oxygen sensor array

Ji Hoon Han, Saeyoung Kim, Jaesung Choi, Sora Kang, Youngmi Kim Pak, James Jungho Pak

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    This paper describes a multi-well-based (24-well) electrochemical dissolved oxygen sensor (m-well ECDO sensor) array on a glass substrate, using Nafion as a solid polymer electrolyte. The designed m- well ECDO sensor array shares working electrodes, reference electrodes, and counter electrodes, to reduce the number of electrode pads to approximately one-fourth of its expected pad numbers (72 to 18), compared to the simply repeated 24 sensors. This m-well ECDO sensor array can simultaneously measure up to six sensors at individual crossing points of the row and column electrodes. The fabricated m-well ECDO sensor array showed good repeatability during five consecutive measurements at intervals of 100 s with a 2.4 μm thick Nafion membrane. The response time was about 10 s when the solution state changed from the air-saturated state to the zero-oxygen state, and the measured output current was about -1 μA and-20 nA in the corresponding states, respectively. The fabricated m-well ECDO sensor array was applied to measure the oxygen consumption rate of C2C12 cells for a cell metabolic study. The ratios of the uncoupled oxygen consumption rate (OCR) to the coupled OCR obtained from three sensors of the fabricated m-well ECDO sensor array were 2.16, 2.22, 2.18, which are higher than 1.71, 1.68, 1.73 obtained from of the XF-24 of Agilent Inc. in 5,000, 10,000, 30,000 cells, respectively. This indicates that using the fabricated m-well ECDO sensor array, variations in DO (dissolved oxygen) concentration due to cellular respiration can be effectively detected, even at low cell numbers.

    Original languageEnglish
    Article number127465
    JournalSensors and Actuators, B: Chemical
    Volume306
    DOIs
    Publication statusPublished - 2020 Mar 1

    Bibliographical note

    Funding Information:
    This work was supported by the R&D program of MOTIE/KEIT. [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells]

    Funding Information:
    This work was supported by the R&D program of MOTIE/KEIT . [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells] Appendix A

    Publisher Copyright:
    © 2019 Elsevier B.V.

    Keywords

    • Cell metabolism
    • Dissolved oxygen sensor
    • Electrochemical sensor
    • Multi-well based sensor array
    • Oxygen consumption rate

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Instrumentation
    • Condensed Matter Physics
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Electrical and Electronic Engineering
    • Materials Chemistry

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