Developmental toxicity of chlorpropham induces pathological changes and vascular irregularities in zebrafish embryos

Jin Young Lee, Hahyun Park, Whasun Lim, Gwonhwa Song

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Chlorpropham is used to prevent sprouting in stored agricultural products. It functions through mitosis inhibition or microtubule assembly inhibition in target organisms including plants, protozoa, and fungi. Although the toxicity ranges of chlorpropham in different organisms are known, specific studies on the environmental contamination and the harmful effects of chlorpropham has not been elucidated. In the present study, we demonstrated that toxicity assays of chlorpropham using zebrafish embryos showed pathological morphology alteration with half the embryos undergoing embryonic death. Fluorescent dye was used in live embryos to identify whether oxidative stress and apoptosis mediated developmental malformation. Specific genes related to apoptosis, ccnd1, ccne1, and cdk6, belonging to cell cycle regulation were downregulated on exposure to sublethal concentrations of chlorpropham. Moreover, vascular morphogenesis, which contributes to the cardiovascular circulatory system, was disrupted by chlorpropham along with decreased expression of specific regulators (flt1, kdr, and vegfaa). These data suggest that environmentally preserved chlorpropham is a potential pollutant in non-target species, especially in aquatic organisms, and emphasizes the need for caution regarding the ecotoxicity of chlorpropham.

Original languageEnglish
Article number108802
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Publication statusPublished - 2020 Oct

Bibliographical note

Funding Information:
This research was supported by National Research Foundation of Korea (NRF) grant funded by Ministry of Science and ICT ( MSIT ), South Korea (grant number: 2018R1C1B6009048 ).


  • Apoptosis
  • Chlorpropham
  • Developmental toxicity
  • Oxidative stress
  • Vascular morphogenesis
  • Zebrafish embryos

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis


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