Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea

A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194^T, was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194^T was observed between 15 and 45 °C (optimally at 30-35 °C) and between pH 6.0 and 9.5 (optimally at pH 7.5-9.0). The predominant fatty acids were 11-methyl C_18:1ω7c, C_16:0, C_18:1ω7c, C_18:0 and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194^T formed a well-defined phyletic lineage within the genus Devosia (with 100% bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other Devosia species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194^T represents a novel species within the genus Devosia, for which the name Devosia geojensis sp. nov. is proposed. The type strain is BD-c194^T (=KCTC 22082^T=DSM 19414^T).