TY - JOUR
T1 - Different conformational families of pyrimidine·purine·pyrimidine triple helices depending on backbone composition
AU - Han, Hogyu
AU - Dervan, Peter B.
N1 - Funding Information:
We are grateful to the National Institutes of Health for grant support.
PY - 1994/7/25
Y1 - 1994/7/25
N2 - Different helical conformations of DNA (D), RNA (R), and DNA-RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA Fe to a single nucleotide on RNA as well as DNA oligonucleotides. Cleavage patterns generated by a localized diffusible oxidant in the major groove on the pyrimidine strand of four purine-pyrimidine double helices consisting of all DNA, all RNA, and the corresponding hybrids reveal that the relative cleavage intensity shifts to the 5′ end of the purine strand increasingly in the order: DD<DR<RD<RR. These results are consistent with models derived from structural studies. In six pyrimidine·purine·pyrimidine triple helices, the altered cleavage patterns of the Watson-Crick pyrimidine strands reveal at least two conformational families: (i) D + DD, R + DD, D + DR, and R + DR and (ii) R + RD and R + RR.
AB - Different helical conformations of DNA (D), RNA (R), and DNA-RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA Fe to a single nucleotide on RNA as well as DNA oligonucleotides. Cleavage patterns generated by a localized diffusible oxidant in the major groove on the pyrimidine strand of four purine-pyrimidine double helices consisting of all DNA, all RNA, and the corresponding hybrids reveal that the relative cleavage intensity shifts to the 5′ end of the purine strand increasingly in the order: DD<DR<RD<RR. These results are consistent with models derived from structural studies. In six pyrimidine·purine·pyrimidine triple helices, the altered cleavage patterns of the Watson-Crick pyrimidine strands reveal at least two conformational families: (i) D + DD, R + DD, D + DR, and R + DR and (ii) R + RD and R + RR.
UR - http://www.scopus.com/inward/record.url?scp=0028095194&partnerID=8YFLogxK
U2 - 10.1093/nar/22.14.2837
DO - 10.1093/nar/22.14.2837
M3 - Article
C2 - 7519770
AN - SCOPUS:0028095194
SN - 0305-1048
VL - 22
SP - 2837
EP - 2844
JO - Nucleic acids research
JF - Nucleic acids research
IS - 14
ER -