Differentiation of human neural progenitor cells on PLGA microfibers

C. M. Hwang, S. K. Kim, L. H. Kim, A. Khademhosseini, S. H. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


In this study, we investigated the application of human embryonic stem cells (hESCs) to neural tissue engineering. hESCs were differentiated to neural progenitor cells and cultured on poly(lactic-co-glycolic acid) (pLGA) microfibers. PLGA microfibers were prepared by using a microfluidic spinning system and aligned in parallel manner by winding the nascent fibers around a rotating frame. Neural progenitor cells were prepared by differentiating embryoid bodies (EBs). Neural progenitors cultured on the PLGA fibers were assessed for further proliferation and differentiation. To verify the expression of neural progenitor markers, cells were immunostained with neuronal specific antibodies. After six-days in culture, neuronal protein expression was confirmed with neuron specific beta III tubulin antibody (Tujl) and glial fibrillary acidic protein (GFAP). After 20-days, cells were immunostained with microfilament associated protein (MAP) and neurofilament. The neurites of differentiated cells cultured for 20 days elongated along the direction of the PLGA microfibers. This result shows the possibility of using PLGA microfibers for neural tissue regeneration as a guidance cue for hESC derived cells.

Original languageEnglish
Title of host publicationNEBEC 2009 - Proceedings of the IEEE 35th Annual Northeast Bioengineering Conference
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Print)9781424443628
Publication statusPublished - 2009
EventIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009 - Boston, MA, United States
Duration: 2009 Apr 32009 Apr 5

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X


OtherIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009
Country/TerritoryUnited States
CityBoston, MA

ASJC Scopus subject areas

  • Bioengineering


Dive into the research topics of 'Differentiation of human neural progenitor cells on PLGA microfibers'. Together they form a unique fingerprint.

Cite this