Diffraction optical tomography using a quantitative phase imaging unit

Kyoohyun Kim; Zahid Yaqoob; Kyeoreh Lee; Jeon Woong Kang; Youngwoon Choi; Poorya Hosseini; Peter T.C. So; Yongkeun Park

doi:10.1364/OL.39.006935

Diffraction optical tomography using a quantitative phase imaging unit

Kyoohyun Kim, Zahid Yaqoob, Kyeoreh Lee, Jeon Woong Kang, Youngwoon Choi, Poorya Hosseini, Peter T.C. So, Yongkeun Park

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.

Original language	English
Pages (from-to)	6935-6938
Number of pages	4
Journal	Optics Letters
Volume	39
Issue number	24
DOIs	https://doi.org/10.1364/OL.39.006935
Publication status	Published - 2014 Dec 15
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.39.006935

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title = "Diffraction optical tomography using a quantitative phase imaging unit",

abstract = "A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.",

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AU - Yaqoob, Zahid

AU - Lee, Kyeoreh

AU - Kang, Jeon Woong

AU - Choi, Youngwoon

AU - Hosseini, Poorya

AU - So, Peter T.C.

AU - Park, Yongkeun

PY - 2014/12/15

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AB - A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.

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Diffraction optical tomography using a quantitative phase imaging unit

Abstract

ASJC Scopus subject areas

UN SDGs

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