Diffraction optical tomography using a quantitative phase imaging unit

Kyoohyun Kim, Zahid Yaqoob, Kyeoreh Lee, Jeon Woong Kang, Youngwoon Choi, Poorya Hosseini, Peter T.C. So, Yongkeun Park

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)


A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.

Original languageEnglish
Pages (from-to)6935-6938
Number of pages4
JournalOptics Letters
Issue number24
Publication statusPublished - 2014 Dec 15
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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