TY - GEN
T1 - Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing
AU - Sandoz, Patrick A.
AU - Coskun, Ahmet F.
AU - Chung, Aram J.
AU - Weaver, Westbrook M.
AU - Adeyiga, Oladunni
AU - Khodadadi, Delaram
AU - Ozcan, Aydogan
AU - Di Carlo, Dino
PY - 2012
Y1 - 2012
N2 - We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.
AB - We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.
KW - Digital elisa
KW - Fluorophore stabilization
KW - Water in oil emulsion
KW - Wide field of view fluorescence imaging
UR - http://www.scopus.com/inward/record.url?scp=84901815104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901815104&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901815104
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 338
EP - 340
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -
