Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing

Patrick A. Sandoz; Ahmet F. Coskun; Aram J. Chung; Westbrook M. Weaver; Oladunni Adeyiga; Delaram Khodadadi; Aydogan Ozcan; Dino Di Carlo

Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing

Patrick A. Sandoz^*
, Ahmet F. Coskun
, Aram J. Chung
, Westbrook M. Weaver
, Oladunni Adeyiga
, Delaram Khodadadi
, Aydogan Ozcan
, Dino Di Carlo

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	Chemical and Biological Microsystems Society
Pages	338-340
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012
Externally published	Yes
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1

Publication series

Name	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/Territory	Japan
City	Okinawa
Period	12/10/28 → 12/11/1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Digital elisa
Fluorophore stabilization
Water in oil emulsion
Wide field of view fluorescence imaging

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Sandoz, P. A., Coskun, A. F., Chung, A. J., Weaver, W. M., Adeyiga, O., Khodadadi, D., Ozcan, A., & Di Carlo, D. (2012). Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 338-340). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. / Sandoz, Patrick A.; Coskun, Ahmet F.; Chung, Aram J. et al.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 338-340 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sandoz, PA, Coskun, AF, Chung, AJ, Weaver, WM, Adeyiga, O, Khodadadi, D, Ozcan, A & Di Carlo, D 2012, Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 338-340, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

Sandoz PA, Coskun AF, Chung AJ, Weaver WM, Adeyiga O, Khodadadi D et al. Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 338-340. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Sandoz, Patrick A. ; Coskun, Ahmet F. ; Chung, Aram J. et al. / Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 338-340 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

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abstract = "We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.",

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AU - Sandoz, Patrick A.

AU - Coskun, Ahmet F.

AU - Chung, Aram J.

AU - Weaver, Westbrook M.

AU - Adeyiga, Oladunni

AU - Khodadadi, Delaram

AU - Ozcan, Aydogan

AU - Di Carlo, Dino

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AB - We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.

KW - Digital elisa

KW - Fluorophore stabilization

KW - Water in oil emulsion

KW - Wide field of view fluorescence imaging

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BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

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ER -

Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing

Abstract

Publication series

Other

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this