Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis

Sera Choi, Maxim Prokchorchik, Hyeonjung Lee, Ravi Gupta, Yoonyoung Lee, Eui Hwan Chung, Buhyeon Cho, Min Sung Kim, Sun Tae Kim, Kee Hoon Sohn

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Plant pathogenic bacteria deliver effectors into plant cells to suppress immunity and promote pathogen survival; however, these effectors can be recognized by plant disease resistance proteins to activate innate immunity. The bacterial acetyltransferase effectors HopZ5 and AvrBsT trigger immunity in Arabidopsis thaliana genotypes lacking SUPPRESSOR OF AVRBST-ELICITED RESISTANCE 1 (SOBER1). Using an Arabidopsis accession, Tscha-1, that naturally lacks functional SOBER1 but is unable to recognize HopZ5, we demonstrated that RESISTANCE TO P. SYRINGAE PV MACULICOLA 1 (RPM1) and RPM1-INTERACTING PROTEIN 4 (RIN4) are indispensable for HopZ5- or AvrBsT-triggered immunity. Remarkably, T166 of RIN4, the phosphorylation of which is induced by AvrB and AvrRpm1, is directly acetylated by HopZ5 and AvrBsT. Furthermore, we demonstrated that the acetylation of RIN4 T166 is required and sufficient for HopZ5- or AvrBsT-triggered RPM1-dependent defense activation. Finally, we showed that SOBER1 interferes with HopZ5- or AvrBsT-triggered immunity by deacetylating RIN4 T166. Collectively, our study elucidates detailed molecular mechanisms underlying the activation and suppression of plant innate immunity triggered by two bacterial acetyltransferases, HopZ5 and AvrBsT, from different bacterial pathogens.

Original languageEnglish
Pages (from-to)1951-1960
Number of pages10
JournalMolecular Plant
Volume14
Issue number11
DOIs
Publication statusPublished - 2021 Nov 1

Bibliographical note

Funding Information:
We thank Dr. Mark Youles, Dr. Mark Benfield, and Dr. Richard Hughes for providing the pOPIN constructs. We also thank Dr. Jay Jayaraman for critically reading the manuscript. We thank Dr. Ho Won Jung for providing bacterial strains. This research was supported by Basic Science Research Programs through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF- 2019R1I1A1A01060108 ) and Korean government (MSIT) ( NRF-2018R1A5A1023599 and NRF-2019R1A2C2084705 ). The authors declare no competing interests

Publisher Copyright:
© 2021 The Author

Keywords

  • NLR
  • acetyltransferase effectors
  • effector-triggered immunity
  • immunity suppressors
  • plant deacetylase
  • plant immunity

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Fingerprint

Dive into the research topics of 'Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis'. Together they form a unique fingerprint.

Cite this