Direct application of supercritical carbon dioxide for the reduction of Cronobacter spp. (Enterobacter sakazakii) in end products of dehydrated powdered infant formula

S. A. Kim; O. Y. Kim; M. S. Rhee

doi:10.3168/jds.2009-2738

Direct application of supercritical carbon dioxide for the reduction of Cronobacter spp. (Enterobacter sakazakii) in end products of dehydrated powdered infant formula

S. A. Kim, O. Y. Kim, M. S. Rhee

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The objective of this study was to develop a viable new method for inactivation of Cronobacter spp. that could be applied directly to dehydrated powdered infant formula (PIF) using supercritical carbon dioxide (SC-CO₂). Samples inoculated with Cronobacter spp. were subjected to SC-CO₂ treatment under various conditions (temperature: 63, 68, and 73°C; pressure: 15, 20, and 25MPa; time: 10, 20, and 30min). The survival of Cronobacter spp. was assayed, as were any changes in the quality of the treated PIF. Inactivation of Cronobacter spp. by SC-CO₂ was enhanced as temperature and pressure conditions increased (>6.32 log₁₀ cfu/g). In a validation assay using low-level inoculation (3.21 log₁₀ cfu/g), treatment at 73°C and 15MPa for 30min, 20MPa for 20 and 30min, or 25MPa for 20 and 30min reduced Cronobacter spp. to undetectable levels, with no recovery of cell viability. There was no significant change in water activity, pH, and color of the treated PIF. Overall, the optimum conditions for elimination of Cronobacter spp. were determined to be 73°C and 20MPa for 20min. These parameters for effective SC-CO₂ treatment are feasibly applicable to end product of dehydrated PIF. The results of our study may contribute to the development of an efficient method for improving the microbiological safety of PIF.

Original language	English
Pages (from-to)	1854-1860
Number of pages	7
Journal	Journal of Dairy Science
Volume	93
Issue number	5
DOIs	https://doi.org/10.3168/jds.2009-2738
Publication status	Published - 2010 May

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korea Government (MOEHRD; KRF-2007-311-F00036 ). The authors thank the Korea University Food Safety Center (Seoul, South Korea) for providing the equipment and facilities.

Keywords

Cronobacter spp.
Dehydrated powdered infant formula
Inactivation
Supercritical carbon dioxide

ASJC Scopus subject areas

Food Science
Animal Science and Zoology
Genetics

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10.3168/jds.2009-2738

Cite this

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title = "Direct application of supercritical carbon dioxide for the reduction of Cronobacter spp. (Enterobacter sakazakii) in end products of dehydrated powdered infant formula",

abstract = "The objective of this study was to develop a viable new method for inactivation of Cronobacter spp. that could be applied directly to dehydrated powdered infant formula (PIF) using supercritical carbon dioxide (SC-CO2). Samples inoculated with Cronobacter spp. were subjected to SC-CO2 treatment under various conditions (temperature: 63, 68, and 73°C; pressure: 15, 20, and 25MPa; time: 10, 20, and 30min). The survival of Cronobacter spp. was assayed, as were any changes in the quality of the treated PIF. Inactivation of Cronobacter spp. by SC-CO2 was enhanced as temperature and pressure conditions increased (>6.32 log10 cfu/g). In a validation assay using low-level inoculation (3.21 log10 cfu/g), treatment at 73°C and 15MPa for 30min, 20MPa for 20 and 30min, or 25MPa for 20 and 30min reduced Cronobacter spp. to undetectable levels, with no recovery of cell viability. There was no significant change in water activity, pH, and color of the treated PIF. Overall, the optimum conditions for elimination of Cronobacter spp. were determined to be 73°C and 20MPa for 20min. These parameters for effective SC-CO2 treatment are feasibly applicable to end product of dehydrated PIF. The results of our study may contribute to the development of an efficient method for improving the microbiological safety of PIF.",

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author = "Kim, {S. A.} and Kim, {O. Y.} and Rhee, {M. S.}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korea Government (MOEHRD; KRF-2007-311-F00036 ). The authors thank the Korea University Food Safety Center (Seoul, South Korea) for providing the equipment and facilities.",

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AU - Rhee, M. S.

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korea Government (MOEHRD; KRF-2007-311-F00036 ). The authors thank the Korea University Food Safety Center (Seoul, South Korea) for providing the equipment and facilities.

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N2 - The objective of this study was to develop a viable new method for inactivation of Cronobacter spp. that could be applied directly to dehydrated powdered infant formula (PIF) using supercritical carbon dioxide (SC-CO2). Samples inoculated with Cronobacter spp. were subjected to SC-CO2 treatment under various conditions (temperature: 63, 68, and 73°C; pressure: 15, 20, and 25MPa; time: 10, 20, and 30min). The survival of Cronobacter spp. was assayed, as were any changes in the quality of the treated PIF. Inactivation of Cronobacter spp. by SC-CO2 was enhanced as temperature and pressure conditions increased (>6.32 log10 cfu/g). In a validation assay using low-level inoculation (3.21 log10 cfu/g), treatment at 73°C and 15MPa for 30min, 20MPa for 20 and 30min, or 25MPa for 20 and 30min reduced Cronobacter spp. to undetectable levels, with no recovery of cell viability. There was no significant change in water activity, pH, and color of the treated PIF. Overall, the optimum conditions for elimination of Cronobacter spp. were determined to be 73°C and 20MPa for 20min. These parameters for effective SC-CO2 treatment are feasibly applicable to end product of dehydrated PIF. The results of our study may contribute to the development of an efficient method for improving the microbiological safety of PIF.

AB - The objective of this study was to develop a viable new method for inactivation of Cronobacter spp. that could be applied directly to dehydrated powdered infant formula (PIF) using supercritical carbon dioxide (SC-CO2). Samples inoculated with Cronobacter spp. were subjected to SC-CO2 treatment under various conditions (temperature: 63, 68, and 73°C; pressure: 15, 20, and 25MPa; time: 10, 20, and 30min). The survival of Cronobacter spp. was assayed, as were any changes in the quality of the treated PIF. Inactivation of Cronobacter spp. by SC-CO2 was enhanced as temperature and pressure conditions increased (>6.32 log10 cfu/g). In a validation assay using low-level inoculation (3.21 log10 cfu/g), treatment at 73°C and 15MPa for 30min, 20MPa for 20 and 30min, or 25MPa for 20 and 30min reduced Cronobacter spp. to undetectable levels, with no recovery of cell viability. There was no significant change in water activity, pH, and color of the treated PIF. Overall, the optimum conditions for elimination of Cronobacter spp. were determined to be 73°C and 20MPa for 20min. These parameters for effective SC-CO2 treatment are feasibly applicable to end product of dehydrated PIF. The results of our study may contribute to the development of an efficient method for improving the microbiological safety of PIF.

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Direct application of supercritical carbon dioxide for the reduction of Cronobacter spp. (Enterobacter sakazakii) in end products of dehydrated powdered infant formula

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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