Direct functionalization of cell-adhesion promoters to hydrogel microparticles synthesized by stop-flow lithography

Wookyoung Jang, Do Yeon Kim, Seok Joon Mun, Jun Hee Choi, Yoon Ho Roh, Ki Wan Bong

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Polyethylene glycol (PEG) hydrogel microparticles generated via stop-flow lithography can be utilized for efficient microparticle-based cell culture processes because of their high biocompatibility, the molecular diffusion capability in the gel structure, and the tunability of their shape and size. However, the typical functionalization process of PEG microparticles with cell-adhesion promoters has inevitable limitations, requiring additional linker molecules and the preconjugation of linkers to cell-adhesion promoters and microparticles. In this study, a simple and direct cell-adhesion promoter functionalization process of the PEG microparticles is presented by use of aza-Michael reaction between remnant unreacted acrylate groups in particles and amine groups in cell-adhesion promoters. On the basis of proposed process, particles are directly conjugated with poly-l-lysine (PLL), a typical cell-adhesion promoter that can electrostatically interact with cellular membranes, in a controllable manner. We demonstrate enhanced cell-adhesion capabilities of the particles along with the increased amount of conjugated PLL in the particles. Furthermore, to validate extended applicability, the particles are directly conjugated with Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, in which RGD sequence is involved in the cell-adhesion behavior of extracellular matrix proteins, including fibronectin. The introduced GRGDS peptides increase the cell-adhesion capacity of the microparticles binding to integrin proteins in cellular membranes.

Original languageEnglish
Pages (from-to)1767-1777
Number of pages11
JournalJournal of Polymer Science
Volume60
Issue number11
DOIs
Publication statusPublished - 2022 Jun 1

Bibliographical note

Funding Information:
Next‐Generation Biogreen 21 Program funded by the Rural Development Administration of the Republic of Korea, Grant/Award Number: PJ016004; Engineering Research Center of Excellence Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning, Grant/Award Number: NRF‐2016R1A5A1010148; Basic Science Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Grant/Award Number: NRF‐2018R1D1A1B07046577 Funding information

Funding Information:
This research was supported by the Basic Science Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF‐2018R1D1A1B07046577), a grant from the Engineering Research Center of Excellence Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF‐2016R1A5A1010148), and the Next‐Generation Biogreen 21 Program (no. PJ016004), Rural Development Administration, Republic of Korea.

Publisher Copyright:
© 2022 The Authors. Journal of Polymer Science published by Wiley Periodicals LLC.

Keywords

  • Michael addition
  • bio-adhesion
  • cell culture
  • hydrogel
  • poly-l-lysine

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Physical and Theoretical Chemistry

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