Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2

Hye Won Park; Hana Jung; Kee Hyun Choi; Ja Hyun Baik; Hyewhon Rhim

doi:10.1016/j.febslet.2010.07.014

Direct interaction and functional coupling between voltage-gated Ca_V1.3 Ca²⁺ channel and GABA_B receptor subunit 2

Hye Won Park, Hana Jung, Kee Hyun Choi, Ja Hyun Baik, Hyewhon Rhim

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Although Ca_V1.2 and Ca_V1.3 are two subtypes of L-type Ca²⁺ channels expressed in the CNS, functions of Ca_V1.3 have not been well elucidated compared to Ca_V1.2. Here, we found that Ca_V1.3-NT associates with GABA_BR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of Ca_V1.3 and GABA_BR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca²⁺-imaging experiments revealed that activation of GABA_BR increases Ca_V1.3 currents and intracellular Ca²⁺ via Ca_V1.3, but not Ca_V1.2. These results show a physical and functional interaction between Ca_V1.3 and GABA_BR, suggesting the potential pivotal roles of Ca_V1.3 in the CNS.

Original language	English
Pages (from-to)	3317-3322
Number of pages	6
Journal	FEBS Letters
Volume	584
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2010.07.014
Publication status	Published - 2010 Aug

Keywords

Baclofen
Intracellular Ca
L-Type Ca channels
Whole-cell patch-clamp

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2010.07.014

Cite this

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title = "Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2",

abstract = "Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.",

keywords = "Baclofen, Intracellular Ca, L-Type Ca channels, Whole-cell patch-clamp",

author = "Park, {Hye Won} and Hana Jung and Choi, {Kee Hyun} and Baik, {Ja Hyun} and Hyewhon Rhim",

note = "Funding Information: We extend our appreciation to Dr. S. Kim for helping a yeast two-hybrid assay. This work was supported by the Mid-Career Researcher Program Grant (No. 20100000343 ), the Pioneer Grant (No. 20100002215 ), and the BRC of the 21st Century Frontier Research Program (No. 2010K000813) from MEST, the Republic of Korea.",

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AU - Park, Hye Won

AU - Jung, Hana

AU - Choi, Kee Hyun

AU - Baik, Ja Hyun

AU - Rhim, Hyewhon

N1 - Funding Information: We extend our appreciation to Dr. S. Kim for helping a yeast two-hybrid assay. This work was supported by the Mid-Career Researcher Program Grant (No. 20100000343 ), the Pioneer Grant (No. 20100002215 ), and the BRC of the 21st Century Frontier Research Program (No. 2010K000813) from MEST, the Republic of Korea.

PY - 2010/8

Y1 - 2010/8

N2 - Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

AB - Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

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