Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2

Hye Won Park, Hana Jung, Kee Hyun Choi, Ja Hyun Baik, Hyewhon Rhim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

Original languageEnglish
Pages (from-to)3317-3322
Number of pages6
JournalFEBS Letters
Volume584
Issue number15
DOIs
Publication statusPublished - 2010 Aug

Bibliographical note

Funding Information:
We extend our appreciation to Dr. S. Kim for helping a yeast two-hybrid assay. This work was supported by the Mid-Career Researcher Program Grant (No. 20100000343 ), the Pioneer Grant (No. 20100002215 ), and the BRC of the 21st Century Frontier Research Program (No. 2010K000813) from MEST, the Republic of Korea.

Keywords

  • Baclofen
  • Intracellular Ca
  • L-Type Ca channels
  • Whole-cell patch-clamp

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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