Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Biochemistry, Genetics and Molecular Biology

• DNA Cleavage 100%
• Cas9 100%
• CRISPR-Cas12A 100%
• Protospacer Adjacent Motif 100%
• Facilitated Diffusion 50%
• Deoxyribonuclease 50%
• RNA 50%
• Ribonucleoprotein 50%
• DNA Strand 50%
• CRISPR 50%
• CRISPR/Cas9 50%
• Genome Editing 50%
• R-Loop 50%
• Acidaminococcus 50%
• DNA Denaturation 50%
• Fluorescence Assay 50%

Keyphrases

• DNA Target 100%
• Target Search 100%
• Target Cleavage 100%
• CRISPR-Cas12a 100%
• Cas12a 100%
• AsCas12a 60%
• DNA Cleavage 40%
• Protospacer Adjacent Motif 40%
• Well-defined 20%
• RNA-based 20%
• Non-target 20%
• Target DNA 20%
• Detailed Mechanism 20%
• Single-molecule Fluorescence 20%
• DNA Molecule 20%
• One-dimensional Diffusion 20%
• DNA Strands 20%
• Ribonucleoprotein 20%
• DNA nuclease 20%
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) 20%
• Fluorescence Assay 20%
• Cas9 Protein 20%
• Genome Editing 20%
• R-loop Formation 20%
• DNA Unwinding 20%