Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Kondapa Naidu Bobba; Anupama Binoy; Seyoung Koo; Divya Nedungadi; Arup Podder; Amit Sharma; Nandita Mishra; Jong Seung Kim; Sankarprasad Bhuniya

doi:10.1039/c9cc01483g

Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Kondapa Naidu Bobba, Anupama Binoy, Seyoung Koo, Divya Nedungadi, Arup Podder, Amit Sharma, Nandita Mishra, Jong Seung Kim, Sankarprasad Bhuniya

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H₂S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H₂S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

Original language	English
Pages (from-to)	6429-6432
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	45
DOIs	https://doi.org/10.1039/c9cc01483g
Publication status	Published - 2019

Bibliographical note

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells",

abstract = "Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.",

author = "Bobba, {Kondapa Naidu} and Anupama Binoy and Seyoung Koo and Divya Nedungadi and Arup Podder and Amit Sharma and Nandita Mishra and Kim, {Jong Seung} and Sankarprasad Bhuniya",

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year = "2019",

doi = "10.1039/c9cc01483g",

language = "English",

volume = "55",

pages = "6429--6432",

journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

AU - Bobba, Kondapa Naidu

AU - Binoy, Anupama

AU - Koo, Seyoung

AU - Nedungadi, Divya

AU - Podder, Arup

AU - Sharma, Amit

AU - Mishra, Nandita

AU - Kim, Jong Seung

AU - Bhuniya, Sankarprasad

PY - 2019

Y1 - 2019

N2 - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

AB - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

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U2 - 10.1039/c9cc01483g

DO - 10.1039/c9cc01483g

M3 - Article

C2 - 31094377

AN - SCOPUS:85066451443

SN - 1359-7345

VL - 55

SP - 6429

EP - 6432

JO - Chemical Communications

JF - Chemical Communications

IS - 45

ER -

Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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