Directional and trans-endothelial migration of mesenchymal stem cell towards SDF-1a gradient on a microfluidic device

Siwan Park, Hwanseok Jang, Gi Seok Jeong, Byung Soo Kim, Yongdoo Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Homing of mesenchymal stem cell (MSC) for tissue regeneration is known to be controlled by a homing factor, stromal cell-derived factor 1α (SDF-1α) gradient released from injury site. Binding of SDF-1α leads to MSC's extravasation and migration towards the infarcted tissue. In this study, a microfluidic device was designed to witness the homing process of MSC towards SDF-1α gradient. SDF-1 antagonist AMD3100 blocked the binding of SDF-1α to the MSC's receptor which led to loss of directional migration. On the other hand, Rock inhibitor, Y-27632 and Rac inhibitor, NSC23766 had no effects on directional migration but decreased MSC's migration distance.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages453-454
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Collagen scaffold
  • Directional migration
  • EC
  • Extravasation
  • MSC
  • Microfluidics
  • SDF-1α

ASJC Scopus subject areas

  • Control and Systems Engineering

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