TY - JOUR
T1 - Discovery and genetic characterization of novel paramyxoviruses related to the genus henipavirus in crocidura species in the republic of Korea
AU - Lee, Seung Ho
AU - Kim, Kijin
AU - Kim, Jongwoo
AU - No, Jin Sun
AU - Park, Kyungmin
AU - Budhathoki, Shailesh
AU - Lee, Seung Ho
AU - Lee, Jingyeong
AU - Cho, Seung Hye
AU - Cho, Seungchan
AU - Lee, Geum Young
AU - Hwang, Jusun
AU - Kim, Heung Chul
AU - Klein, Terry A.
AU - Uhm, Chang Sub
AU - Kim, Won Keun
AU - Song, Jin Won
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10
Y1 - 2021/10
N2 - Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were examined for the prevalence of paramyxovirus infections. Paramyxovirus RNA was observed in 26 (22.6%) shrews collected at five trapping sites, Republic of Korea. Herein, we report two genetically distinct novel paramyxoviruses (genus: Henipavirus): Gamak virus (GAKV) and Daeryong virus (DARV) isolated from C. lasiura and C. shantungensis, respectively. Two GAKVs and one DARV were nearly completely sequenced using next-generation sequencing. GAKV and DARV contain six genes (3’-N-P-M-F-G-L-5´) with genome sizes of 18,460 nucleotides and 19,471 nucleotides, respectively. The phylogenetic inference demonstrated that GAKV and DARV form independent genetic lineages of Henipavirus in Crocidura species. GAKV-infected human lung epithelial cells elicited the induction of type I/III interferons, interferon-stimulated genes, and proinflammatory cytokines. In conclusion, this study contributes further understandings of the molecular prevalence, genetic characteristics and diversity, and zoonotic potential of novel para-myxoviruses in shrews.
AB - Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were examined for the prevalence of paramyxovirus infections. Paramyxovirus RNA was observed in 26 (22.6%) shrews collected at five trapping sites, Republic of Korea. Herein, we report two genetically distinct novel paramyxoviruses (genus: Henipavirus): Gamak virus (GAKV) and Daeryong virus (DARV) isolated from C. lasiura and C. shantungensis, respectively. Two GAKVs and one DARV were nearly completely sequenced using next-generation sequencing. GAKV and DARV contain six genes (3’-N-P-M-F-G-L-5´) with genome sizes of 18,460 nucleotides and 19,471 nucleotides, respectively. The phylogenetic inference demonstrated that GAKV and DARV form independent genetic lineages of Henipavirus in Crocidura species. GAKV-infected human lung epithelial cells elicited the induction of type I/III interferons, interferon-stimulated genes, and proinflammatory cytokines. In conclusion, this study contributes further understandings of the molecular prevalence, genetic characteristics and diversity, and zoonotic potential of novel para-myxoviruses in shrews.
KW - Crocidura paramyxovirus
KW - Genetic characterization and diversity
KW - Next-generation sequencing
KW - Novel virus discovery
KW - Potential zoonosis
UR - http://www.scopus.com/inward/record.url?scp=85116972177&partnerID=8YFLogxK
U2 - 10.3390/v13102020
DO - 10.3390/v13102020
M3 - Article
C2 - 34696450
AN - SCOPUS:85116972177
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 10
M1 - 2020
ER -