Discovery of Novel Pseudomonas putida Flavin-Binding Fluorescent Protein Variants with Significantly Improved Quantum Yield

Sanghwan Ko, Sanghwan Ko, Hyunwoo Jeon, Sanghan Yoon, Munsu Kyung, Hyungdon Yun, Jung Hyun Na, Sang Taek Jung

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Oxygen-independent, flavin-binding fluorescent proteins (FbFPs) are emerging as alternatives to green fluorescent protein (GFP), which has limited applicability in studying anaerobic microorganisms, such as human gastrointestinal bacteria, which grow in oxygen-deficient environments. However, the utility of these FbFPs has been compromised because of their poor fluorescence emission. To overcome this limitation, we have employed a high-throughput library screening strategy and engineered an FbFP derived from Pseudomonas putida (SB2) for enhanced quantum yield. Of the resulting SB2 variants, KOFP-7 exhibited a significantly improved quantum yield (0.61) compared to other reported engineered FbFPs, which was even higher than that of enhanced GFP (EGFP, 0.60), with significantly enhanced tolerance against a strong reducing agent.

Original languageEnglish
Pages (from-to)5873-5879
Number of pages7
JournalJournal of agricultural and food chemistry
Volume68
Issue number21
DOIs
Publication statusPublished - 2020 May 27

Bibliographical note

Funding Information:
This work was supported by a Korea University grant and grants from the Basic Science Research Program (2019R1F1A1059834 and 2019R1A4A1029000), the Bio and Medical Technology Development Program (2017M3A9C8060541), and the Pioneer Research Center Program (2014M3C1A3051460) through the National Research Foundation of Korea funded by the Ministry of Science and ICT.

Publisher Copyright:
© 2020 American Chemical Society.

Keywords

  • flavin-binding fluorescent protein
  • fluorescence intensity
  • quantum yield

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

Fingerprint

Dive into the research topics of 'Discovery of Novel Pseudomonas putida Flavin-Binding Fluorescent Protein Variants with Significantly Improved Quantum Yield'. Together they form a unique fingerprint.

Cite this