Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents

Jiho Min; Eun Jin Kim; Robert A. Larossa; Man Bock Gu

doi:10.1016/S1383-5718(99)00059-5

Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents

Jiho Min, Eun Jin Kim, Robert A. Larossa, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

89 Citations (Scopus)

Abstract

The recombinant Escherichia coli strain DPD2794 containing a recA::luxCDABE fusion is used to detect genotoxicity of various chemicals. Genotoxic agents were previously categorized into two groups, Direct DNA Damaging (DDD) agents and Indirect DNA Damaging (IDD) agents; these two groups have been distinguished with this strain. Minimum detectable concentrations of the DDD agents were about one to five orders of magnitude lower than those of the IDD agents. The response patterns of this strain to DDD agents differed from those to IDD agents in terms of kinetics and the forms of the dose-dependent response. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	61-68
Number of pages	8
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	442
Issue number	2
DOIs	https://doi.org/10.1016/S1383-5718(99)00059-5
Publication status	Published - 1999 Jun 25
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Korea Minister of Health and Welfare (Grant # HMP-98-F-1-0003) and partially supported by the Korea Science and Engineering Foundation (KOSEF) (Grant # 971-1106-042-2). Authors are grateful for their support.

Keywords

Direct and indirect DNA damage
Genotoxicity
recA promoter

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

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10.1016/S1383-5718(99)00059-5

Cite this

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abstract = "The recombinant Escherichia coli strain DPD2794 containing a recA::luxCDABE fusion is used to detect genotoxicity of various chemicals. Genotoxic agents were previously categorized into two groups, Direct DNA Damaging (DDD) agents and Indirect DNA Damaging (IDD) agents; these two groups have been distinguished with this strain. Minimum detectable concentrations of the DDD agents were about one to five orders of magnitude lower than those of the IDD agents. The response patterns of this strain to DDD agents differed from those to IDD agents in terms of kinetics and the forms of the dose-dependent response. Copyright (C) 1999 Elsevier Science B.V.",

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Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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