Distinct role of IL-3 promoter and enhancer region in murine mast cells

Chang Bo Ko, Bok Soo Lee, Seok Ho Cha, Donggeun Sul, Sang Gi Paik, Hyung Sik Kang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Crosslinking of Fcε receptor on mast cells induces IL-3 gene expression with the concentration dependent of intracellular calcium, but its regulatory mechanism remains unclear. Here, we found that phorbol 12-myristate 13-acetate (PMA) alone did not induce IL-3 gene expression, but potentiated A23187-induced IL-3 gene expression. Interestingly, the A23187-induced IL-3 promoter activity was suppressed by PMA, but it was enhanced when IL-3 promoter contained enhancer region, a DH site. While IL-3 mRNA expression was increased by A23187 and PMA in a dose-dependent manner, the promoter activity appeared all or none in all doses of A23187 and PMA. IL-3 promoter region between -293 and -150 bp was responsible for A23187-induced gene expression and PMA- or cyclosporin A (CsA)-mediated suppression. Taken together, IL-3 gene expression was primarily regulated at the transcriptional level, which was differentially controlled by a restricted promoter and enhancer region.

Original languageEnglish
Pages (from-to)1569-1576
Number of pages8
JournalMolecular Immunology
Volume44
Issue number7
DOIs
Publication statusPublished - 2007 Mar

Bibliographical note

Funding Information:
We thank Dr. C. Moroni for providing mast cell lines and critical reading of the manuscript with helpful comments. This work was supported by grants from the Korea Science Engineering Foundation through Research Center for Women's Diseases (R11-2005-017-02002) and the Korea Research Foundation (KRF-2004-005-E00015).

Keywords

  • Cytokines
  • Gene regulation
  • IL-3
  • Intracellular calcium
  • Mast cells
  • Transcription

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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