Diterpenoids as potential anti-inflammatory agents from Ajuga pantantha

Wenpei Liu, Ziteng Song, Huimei Wang, Xueyuan Yang, Elizabeth Joubert, Jie Zhang, Shen Li, Muhetaer Tuerhong, Munira Abudukeremu, Jin Jin, Jing Xu, Dongho Lee, Yuanqiang Guo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A phytochemical survey to obtain bioactive natural products from Ajuga pantantha afforded five new neo-clerodane diterpenoids (1–5). The structures were established by analysis of their NMR spectroscopic data, and electronic circular dichroism calculations were applied to define their absolute configurations. Compounds 2 and 5 were found to have the property of inhibiting NO production (IC50 values < 40 μM). Molecular docking and Western blotting were used to study the mechanism of anti-inflammatory action. Furthermore, compound 5 with the highest activity was tested for its in vivo anti-inflammatory effects using a zebrafish model.

Original languageEnglish
Article number103966
JournalBioorganic Chemistry
Volume101
DOIs
Publication statusPublished - 2020 Aug

Bibliographical note

Funding Information:
This research was financially supported by the National Key Research and Development Program of China (No. 2018YFA0507204), the National Natural Science Foundation of China (Nos. U1703107, U1801288, and 21665013), Hundred Young Academic Leaders Program of Nankai University, the Natural Science Foundation of Tianjin (No. 19JCYBJC28100), the State Key Laboratory of Drug Research (No. SIMM2004KF-13), the open project of Key Laboratory of Xinjiang Uygur Autonomous Region (Nos. 2015KL030 and 2017D04019), and the Scientific Research Program of the Higher Education Institution of Xinjiang Uygur Autonomous Region (No. XJEDU20171010).

Funding Information:
This research was financially supported by the National Key Research and Development Program of China (No. 2018YFA0507204 ), the National Natural Science Foundation of China (Nos. U1703107 , U1801288 , and 21665013 ), Hundred Young Academic Leaders Program of Nankai University , the Natural Science Foundation of Tianjin (No. 19JCYBJC28100 ), the State Key Laboratory of Drug Research (No. SIMM2004KF-13 ), the open project of Key Laboratory of Xinjiang Uygur Autonomous Region (Nos. 2015KL030 and 2017D04019 ), and the Scientific Research Program of the Higher Education Institution of Xinjiang Uygur Autonomous Region (No. XJEDU20171010).

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

  • Ajuga pantantha
  • Diterpenoids
  • Inflammation
  • Molecular docking
  • Nitric oxide inhibitory effects
  • Zebrafish model

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

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