Abstract
A phytochemical survey to obtain bioactive natural products from Ajuga pantantha afforded five new neo-clerodane diterpenoids (1–5). The structures were established by analysis of their NMR spectroscopic data, and electronic circular dichroism calculations were applied to define their absolute configurations. Compounds 2 and 5 were found to have the property of inhibiting NO production (IC50 values < 40 μM). Molecular docking and Western blotting were used to study the mechanism of anti-inflammatory action. Furthermore, compound 5 with the highest activity was tested for its in vivo anti-inflammatory effects using a zebrafish model.
Original language | English |
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Article number | 103966 |
Journal | Bioorganic Chemistry |
Volume | 101 |
DOIs | |
Publication status | Published - 2020 Aug |
Bibliographical note
Publisher Copyright:© 2020 Elsevier Inc.
Keywords
- Ajuga pantantha
- Diterpenoids
- Inflammation
- Molecular docking
- Nitric oxide inhibitory effects
- Zebrafish model
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Drug Discovery
- Organic Chemistry