Diterpenoids from the leaves of Casearia kurzii showing cytotoxic activities

Yue Liang; Qi Zhang; Xueyuan Yang; Ying Li; Xuke Zhang; Yuhao Li; Qing Du; Da Qing Jin; Jianlin Cui; Namrita Lall; Muhetaer Tuerhong; Dongho Lee; Munira Abudukeremu; Jing Xu; Ling Shuai; Yuanqiang Guo

doi:10.1016/j.bioorg.2020.103741

Diterpenoids from the leaves of Casearia kurzii showing cytotoxic activities

Yue Liang, Qi Zhang, Xueyuan Yang, Ying Li, Xuke Zhang, Yuhao Li, Qing Du, Da Qing Jin, Jianlin Cui, Namrita Lall, Muhetaer Tuerhong, Dongho Lee, Munira Abudukeremu, Jing Xu, Ling Shuai, Yuanqiang Guo

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A phytochemical investigation to obtain bioactive substances as lead compounds or agents for cancer led to the obtainment of six new and two known clerodane diterpenoids from the leaves of Casearia kurzii. Their structures were elucidated using NMR techniques and electronic circular dichroism (ECD) calculations. The subsequent biological cytotoxicity evaluation of these isolates toward human lung cancer A549, human cervical cancer HeLa, human chronic myeloid leukemia K562, and human hepatocellular carcinoma HepG2 was carried out. The most active compound 4 with an IC₅₀ value of 9.7 μM against HepG2 cells was selected to examine the cytotoxic mechanism, which induced the apoptosis and arrested the HepG2 cell cycle at S stage. The in vivo zebrafish experiments revealed that compound 4 had the property of inhibiting tumor proliferation and migration.

Original language	English
Article number	103741
Journal	Bioorganic Chemistry
Volume	98
DOIs	https://doi.org/10.1016/j.bioorg.2020.103741
Publication status	Published - 2020 May

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

Apoptosis
Casearia kurzii
Cell cycle
Clerodane diterpenoids
Cytotoxic activities
Zebrafish xenograft model

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Drug Discovery
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bioorg.2020.103741

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title = "Diterpenoids from the leaves of Casearia kurzii showing cytotoxic activities",

abstract = "A phytochemical investigation to obtain bioactive substances as lead compounds or agents for cancer led to the obtainment of six new and two known clerodane diterpenoids from the leaves of Casearia kurzii. Their structures were elucidated using NMR techniques and electronic circular dichroism (ECD) calculations. The subsequent biological cytotoxicity evaluation of these isolates toward human lung cancer A549, human cervical cancer HeLa, human chronic myeloid leukemia K562, and human hepatocellular carcinoma HepG2 was carried out. The most active compound 4 with an IC50 value of 9.7 μM against HepG2 cells was selected to examine the cytotoxic mechanism, which induced the apoptosis and arrested the HepG2 cell cycle at S stage. The in vivo zebrafish experiments revealed that compound 4 had the property of inhibiting tumor proliferation and migration.",

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author = "Yue Liang and Qi Zhang and Xueyuan Yang and Ying Li and Xuke Zhang and Yuhao Li and Qing Du and Jin, {Da Qing} and Jianlin Cui and Namrita Lall and Muhetaer Tuerhong and Dongho Lee and Munira Abudukeremu and Jing Xu and Ling Shuai and Yuanqiang Guo",

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year = "2020",

month = may,

doi = "10.1016/j.bioorg.2020.103741",

language = "English",

volume = "98",

journal = "Bioorganic Chemistry",

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AU - Liang, Yue

AU - Zhang, Qi

AU - Yang, Xueyuan

AU - Li, Ying

AU - Zhang, Xuke

AU - Li, Yuhao

AU - Du, Qing

AU - Jin, Da Qing

AU - Cui, Jianlin

AU - Lall, Namrita

AU - Tuerhong, Muhetaer

AU - Lee, Dongho

AU - Abudukeremu, Munira

AU - Xu, Jing

AU - Shuai, Ling

AU - Guo, Yuanqiang

PY - 2020/5

Y1 - 2020/5

N2 - A phytochemical investigation to obtain bioactive substances as lead compounds or agents for cancer led to the obtainment of six new and two known clerodane diterpenoids from the leaves of Casearia kurzii. Their structures were elucidated using NMR techniques and electronic circular dichroism (ECD) calculations. The subsequent biological cytotoxicity evaluation of these isolates toward human lung cancer A549, human cervical cancer HeLa, human chronic myeloid leukemia K562, and human hepatocellular carcinoma HepG2 was carried out. The most active compound 4 with an IC50 value of 9.7 μM against HepG2 cells was selected to examine the cytotoxic mechanism, which induced the apoptosis and arrested the HepG2 cell cycle at S stage. The in vivo zebrafish experiments revealed that compound 4 had the property of inhibiting tumor proliferation and migration.

AB - A phytochemical investigation to obtain bioactive substances as lead compounds or agents for cancer led to the obtainment of six new and two known clerodane diterpenoids from the leaves of Casearia kurzii. Their structures were elucidated using NMR techniques and electronic circular dichroism (ECD) calculations. The subsequent biological cytotoxicity evaluation of these isolates toward human lung cancer A549, human cervical cancer HeLa, human chronic myeloid leukemia K562, and human hepatocellular carcinoma HepG2 was carried out. The most active compound 4 with an IC50 value of 9.7 μM against HepG2 cells was selected to examine the cytotoxic mechanism, which induced the apoptosis and arrested the HepG2 cell cycle at S stage. The in vivo zebrafish experiments revealed that compound 4 had the property of inhibiting tumor proliferation and migration.

KW - Apoptosis

KW - Casearia kurzii

KW - Cell cycle

KW - Clerodane diterpenoids

KW - Cytotoxic activities

KW - Zebrafish xenograft model

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DO - 10.1016/j.bioorg.2020.103741

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AN - SCOPUS:85081960958

SN - 0045-2068

VL - 98

JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

M1 - 103741

ER -

Diterpenoids from the leaves of Casearia kurzii showing cytotoxic activities

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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