TY - JOUR
T1 - DNA barcode library and its efficacy for identifying food-associated insect pests in Korea
AU - Cho, Seo Young
AU - Suh, Kyong In
AU - Bae, Yeon Jae
PY - 2013/9
Y1 - 2013/9
N2 - Food-associated insect pests are of great economic and hygienic importance. However, their identification requires expert knowledge and excessive time. Such pests are discovered in food as body parts or immature stages, which further complicates the identification process. In this study, we constructed a DNA barcode dataset of insect pests that can be detected in food. We also tested the efficacy of these DNA barcode sequences for identifying food-associated insect pests. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed from 55 species of food-associated insect pests in Korea. The results indicated that this portion of the COI gene effectively discriminated >90% of the food-associated insect pests. Mean genetic divergences among individuals belonging to one species/between species belonging to one genus of the five orders, Blattaria, Coleoptera, Hymenoptera, Lepidoptera and Diptera, were 0.59%/13.18%, 0.84%/20.10%, 0.02%/22.61%, 0.24%/3.48% and 0.17%/15.90%, respectively. In conclusion, we established the first DNA barcode dataset and confirmed its efficiency for identifying food-associated insect pests in Korea.
AB - Food-associated insect pests are of great economic and hygienic importance. However, their identification requires expert knowledge and excessive time. Such pests are discovered in food as body parts or immature stages, which further complicates the identification process. In this study, we constructed a DNA barcode dataset of insect pests that can be detected in food. We also tested the efficacy of these DNA barcode sequences for identifying food-associated insect pests. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed from 55 species of food-associated insect pests in Korea. The results indicated that this portion of the COI gene effectively discriminated >90% of the food-associated insect pests. Mean genetic divergences among individuals belonging to one species/between species belonging to one genus of the five orders, Blattaria, Coleoptera, Hymenoptera, Lepidoptera and Diptera, were 0.59%/13.18%, 0.84%/20.10%, 0.02%/22.61%, 0.24%/3.48% and 0.17%/15.90%, respectively. In conclusion, we established the first DNA barcode dataset and confirmed its efficiency for identifying food-associated insect pests in Korea.
KW - Cytochrome c oxidase subunit I gene
KW - DNA barcode
KW - Food-associated insect pests
KW - Korea
UR - http://www.scopus.com/inward/record.url?scp=84884792650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884792650&partnerID=8YFLogxK
U2 - 10.1111/1748-5967.12034
DO - 10.1111/1748-5967.12034
M3 - Article
AN - SCOPUS:84884792650
SN - 1748-5967
VL - 43
SP - 253
EP - 261
JO - Entomological Research
JF - Entomological Research
IS - 5
ER -