DNA methylation patterns of the rat gamma-glutamyl transpeptidase gene in embryonic, adult and neoplastic liver

Ja Hyun Baik; Susan Griffiths; Galicia Giuili; Margaret Manson; Sylvie Siegrist; Georges Guellaen

doi:10.1093/carcin/12.6.1035

DNA methylation patterns of the rat gamma-glutamyl transpeptidase gene in embryonic, adult and neoplastic liver

Ja Hyun Baik, Susan Griffiths, Galicia Giuili, Margaret Manson, Sylvie Siegrist, Georges Guellaen

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The methylation status of the rat gamma-glutamyl transpeptidase (GGT) gene was investigated during liver development and hepatocarcinogenesis. The analysis with the restriction enzymes MspI/HpaII revealed that, during ontogeny, there is a progressive methylation of the GGT gene that coincides with a progressive decrease in GGT activity. Thus, there is an inverse correlation between methylation and expression of the GGT gene, suggesting a role for DNA methylation in the regulation of the gene during normal differentiation. The methylation patterns of the GGT gene in liver tumours induced by aflatoxin B₁ exhibit heterogeneity. Nevertheless, a band of 5.7 KB was observed in all the DNA samples from aflatoxin B₁-induced tumours which was not present in control liver DNA. The specificity of the DNA methylation changes was assessed using nafenopin, which induces hepatic tumours without elevation of GGT activity. We conclude that, during hepatocarcinogenesis, there is a modification of the DNA methylation pattern of the GGT gene, but there is no simple correlation with GGT activity. In no case was the GGT gene methylation in hepatocarcinogenesis found to be equivalent to the pattern observed in fetal liver. Thus if methylation is involved in the regulation of GGT gene transcription, the mechanisms must be different in fetal liver and hepatocarcinoma.

Original language	English
Pages (from-to)	1035-1040
Number of pages	6
Journal	Carcinogenesis
Volume	12
Issue number	6
DOIs	https://doi.org/10.1093/carcin/12.6.1035
Publication status	Published - 1991 Jun
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Dr A.G Smith and Dr B.G.Lake for providing tissue samples from hexachlorobenzene-, diethylnitrosamine-and nafenopin-treated animals and for helpful discussions. We thank Dr Y.Laperche and Dr R.Barouki for their most helpful comments and L.Rosario and E.Grandvilliers for their skilful secretarial assistance. Susan Griffiths was funded by the Association for International Cancer Research. This work was supported by the CNAMTS.

Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/carcin/12.6.1035

Cite this

@article{7c55a6fd185344cdbe1dddd46524703a,

title = "DNA methylation patterns of the rat gamma-glutamyl transpeptidase gene in embryonic, adult and neoplastic liver",

abstract = "The methylation status of the rat gamma-glutamyl transpeptidase (GGT) gene was investigated during liver development and hepatocarcinogenesis. The analysis with the restriction enzymes MspI/HpaII revealed that, during ontogeny, there is a progressive methylation of the GGT gene that coincides with a progressive decrease in GGT activity. Thus, there is an inverse correlation between methylation and expression of the GGT gene, suggesting a role for DNA methylation in the regulation of the gene during normal differentiation. The methylation patterns of the GGT gene in liver tumours induced by aflatoxin B1 exhibit heterogeneity. Nevertheless, a band of 5.7 KB was observed in all the DNA samples from aflatoxin B1-induced tumours which was not present in control liver DNA. The specificity of the DNA methylation changes was assessed using nafenopin, which induces hepatic tumours without elevation of GGT activity. We conclude that, during hepatocarcinogenesis, there is a modification of the DNA methylation pattern of the GGT gene, but there is no simple correlation with GGT activity. In no case was the GGT gene methylation in hepatocarcinogenesis found to be equivalent to the pattern observed in fetal liver. Thus if methylation is involved in the regulation of GGT gene transcription, the mechanisms must be different in fetal liver and hepatocarcinoma.",

author = "Baik, {Ja Hyun} and Susan Griffiths and Galicia Giuili and Margaret Manson and Sylvie Siegrist and Georges Guellaen",

note = "Funding Information: The authors would like to thank Dr A.G Smith and Dr B.G.Lake for providing tissue samples from hexachlorobenzene-, diethylnitrosamine-and nafenopin-treated animals and for helpful discussions. We thank Dr Y.Laperche and Dr R.Barouki for their most helpful comments and L.Rosario and E.Grandvilliers for their skilful secretarial assistance. Susan Griffiths was funded by the Association for International Cancer Research. This work was supported by the CNAMTS. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "1991",

month = jun,

doi = "10.1093/carcin/12.6.1035",

language = "English",

volume = "12",

pages = "1035--1040",

journal = "Carcinogenesis",

issn = "0143-3334",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - DNA methylation patterns of the rat gamma-glutamyl transpeptidase gene in embryonic, adult and neoplastic liver

AU - Baik, Ja Hyun

AU - Griffiths, Susan

AU - Giuili, Galicia

AU - Manson, Margaret

AU - Siegrist, Sylvie

AU - Guellaen, Georges

N1 - Funding Information: The authors would like to thank Dr A.G Smith and Dr B.G.Lake for providing tissue samples from hexachlorobenzene-, diethylnitrosamine-and nafenopin-treated animals and for helpful discussions. We thank Dr Y.Laperche and Dr R.Barouki for their most helpful comments and L.Rosario and E.Grandvilliers for their skilful secretarial assistance. Susan Griffiths was funded by the Association for International Cancer Research. This work was supported by the CNAMTS. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 1991/6

Y1 - 1991/6

N2 - The methylation status of the rat gamma-glutamyl transpeptidase (GGT) gene was investigated during liver development and hepatocarcinogenesis. The analysis with the restriction enzymes MspI/HpaII revealed that, during ontogeny, there is a progressive methylation of the GGT gene that coincides with a progressive decrease in GGT activity. Thus, there is an inverse correlation between methylation and expression of the GGT gene, suggesting a role for DNA methylation in the regulation of the gene during normal differentiation. The methylation patterns of the GGT gene in liver tumours induced by aflatoxin B1 exhibit heterogeneity. Nevertheless, a band of 5.7 KB was observed in all the DNA samples from aflatoxin B1-induced tumours which was not present in control liver DNA. The specificity of the DNA methylation changes was assessed using nafenopin, which induces hepatic tumours without elevation of GGT activity. We conclude that, during hepatocarcinogenesis, there is a modification of the DNA methylation pattern of the GGT gene, but there is no simple correlation with GGT activity. In no case was the GGT gene methylation in hepatocarcinogenesis found to be equivalent to the pattern observed in fetal liver. Thus if methylation is involved in the regulation of GGT gene transcription, the mechanisms must be different in fetal liver and hepatocarcinoma.

AB - The methylation status of the rat gamma-glutamyl transpeptidase (GGT) gene was investigated during liver development and hepatocarcinogenesis. The analysis with the restriction enzymes MspI/HpaII revealed that, during ontogeny, there is a progressive methylation of the GGT gene that coincides with a progressive decrease in GGT activity. Thus, there is an inverse correlation between methylation and expression of the GGT gene, suggesting a role for DNA methylation in the regulation of the gene during normal differentiation. The methylation patterns of the GGT gene in liver tumours induced by aflatoxin B1 exhibit heterogeneity. Nevertheless, a band of 5.7 KB was observed in all the DNA samples from aflatoxin B1-induced tumours which was not present in control liver DNA. The specificity of the DNA methylation changes was assessed using nafenopin, which induces hepatic tumours without elevation of GGT activity. We conclude that, during hepatocarcinogenesis, there is a modification of the DNA methylation pattern of the GGT gene, but there is no simple correlation with GGT activity. In no case was the GGT gene methylation in hepatocarcinogenesis found to be equivalent to the pattern observed in fetal liver. Thus if methylation is involved in the regulation of GGT gene transcription, the mechanisms must be different in fetal liver and hepatocarcinoma.

UR - http://www.scopus.com/inward/record.url?scp=0025762322&partnerID=8YFLogxK

U2 - 10.1093/carcin/12.6.1035

DO - 10.1093/carcin/12.6.1035

M3 - Article

C2 - 1675160

AN - SCOPUS:0025762322

SN - 0143-3334

VL - 12

SP - 1035

EP - 1040

JO - Carcinogenesis

JF - Carcinogenesis

IS - 6

ER -

DNA methylation patterns of the rat gamma-glutamyl transpeptidase gene in embryonic, adult and neoplastic liver

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this