DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells

Min Kyu Oh; Daniels R. Scoles; Stefan M. Pulst

doi:10.1007/BF02989827

DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells

Min Kyu Oh, Daniels R. Scoles, Stefan M. Pulst

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.

Original language	English
Pages (from-to)	444-450
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/BF02989827
Publication status	Published - 2005

Bibliographical note

Funding Information:
^ÅâåçïäÉÇÖÉãÉåíë The authors thank Cedars Sinai Medical Center microarray core facility for performing the microar-ray experiment. This work was supported by the Korea Research Foundation, KRF-2004-005-D00057, and the Carmen and Louis Warschaw endowment for neurology, grants DAMD 17-99-1-9548 and 01-01-0703 from the USAMRAA, and grant R01 NS37883.

Keywords

Epidermal growth factor (EGF)
Expression profiling
Immediate response
Oligonucleotide microarray
Schwannoma cell line

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

Access to Document

10.1007/BF02989827

Cite this

@article{6b4487f8fa454280bb0280a211d8b089,

title = "DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells",

abstract = "Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.",

keywords = "Epidermal growth factor (EGF), Expression profiling, Immediate response, Oligonucleotide microarray, Schwannoma cell line",

author = "Oh, {Min Kyu} and Scoles, {Daniels R.} and Pulst, {Stefan M.}",

note = "Funding Information: ^{\AA}{\^a}{\aa}{\c c}{\"i}{\"a}{\'E}{\c C}{\"O}{\'E}{\~a}{\'E}{\aa}{\'i}{\"e} The authors thank Cedars Sinai Medical Center microarray core facility for performing the microar-ray experiment. This work was supported by the Korea Research Foundation, KRF-2004-005-D00057, and the Carmen and Louis Warschaw endowment for neurology, grants DAMD 17-99-1-9548 and 01-01-0703 from the USAMRAA, and grant R01 NS37883.",

year = "2005",

doi = "10.1007/BF02989827",

language = "English",

volume = "10",

pages = "444--450",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Korean Society for Biotechnology and Bioengineering",

number = "5",

}

TY - JOUR

T1 - DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells

AU - Oh, Min Kyu

AU - Scoles, Daniels R.

AU - Pulst, Stefan M.

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåíë The authors thank Cedars Sinai Medical Center microarray core facility for performing the microar-ray experiment. This work was supported by the Korea Research Foundation, KRF-2004-005-D00057, and the Carmen and Louis Warschaw endowment for neurology, grants DAMD 17-99-1-9548 and 01-01-0703 from the USAMRAA, and grant R01 NS37883.

PY - 2005

Y1 - 2005

N2 - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.

AB - Epidermal growth factor (EGF) activates many intracellular effector molecules, which subsequently influence the expression levels of many genes involved in cell growth, apoptosis and signal transduction, etc. In this study, the early response of gene expressions due to EGF treatment was monitored using oligonucleotide DNA microarrays in rat schwannoma cell lines. An immunoblotting experiment showed the successful activation of EGF receptors and an effector protein, STAT5, due to EGF treatment. The microarray study showed that 35 genes were significantly induced and 2 were repressed within 60 min after the treatment. The list of induced genes included early growth response 1, suppressor of cytokine signaling 3, c-fos, interferon regulatory factor 1 and early growth response 2, etc. According to the microarray data, six of these were induced by more than 10-fold, and showed at least two different induction patterns, indicating complicated regulatory mechanisms in the EGF signal transduction.

KW - Epidermal growth factor (EGF)

KW - Expression profiling

KW - Immediate response

KW - Oligonucleotide microarray

KW - Schwannoma cell line

UR - http://www.scopus.com/inward/record.url?scp=27744443635&partnerID=8YFLogxK

U2 - 10.1007/BF02989827

DO - 10.1007/BF02989827

M3 - Article

AN - SCOPUS:27744443635

SN - 1226-8372

VL - 10

SP - 444

EP - 450

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 5

ER -

DNA Microarray analysis of immediate response to EGF treatment in rat schwannoma cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this