Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

Yoon Hee Cho; Dae Sung Kim; Peter Geon Kim; Yong Sup Hwang; Myung Soo Cho; Shin Yong Moon; Dong Wook Kim; Jin Woo Chang

doi:10.1016/j.bbrc.2005.12.140

Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

Yoon Hee Cho, Dae Sung Kim, Peter Geon Kim, Yong Sup Hwang, Myung Soo Cho, Shin Yong Moon, Dong Wook Kim, Jin Woo Chang

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

Original language	English
Pages (from-to)	6-12
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	341
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.12.140
Publication status	Published - 2006 Mar 3
Externally published	Yes

Keywords

Behavior recovery
Differentiation
Dopamine neurons
ES cells
Parkinson's disease

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2005.12.140

Cite this

@article{8f1b90a62bf44e66b172a7138e50bdfc,

title = "Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model",

abstract = "To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with na{\"i}ve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.",

keywords = "Behavior recovery, Differentiation, Dopamine neurons, ES cells, Parkinson's disease",

author = "Cho, {Yoon Hee} and Kim, {Dae Sung} and Kim, {Peter Geon} and Hwang, {Yong Sup} and Cho, {Myung Soo} and Moon, {Shin Yong} and Kim, {Dong Wook} and Chang, {Jin Woo}",

note = "Funding Information: This research was supported by a Grant (03-PJ1-PG1-CH07-0004) from the Korea Health 21 R&D Project, Ministry of Health & Welfare and Grants (codes: SC1020, SC2140, and SC2160) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are grateful to Dr. Kwang-Soo Kim at Harvard Medical School/Mclean Hospital for the gift of N2 cells. ",

year = "2006",

month = mar,

day = "3",

doi = "10.1016/j.bbrc.2005.12.140",

language = "English",

volume = "341",

pages = "6--12",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

AU - Cho, Yoon Hee

AU - Kim, Dae Sung

AU - Kim, Peter Geon

AU - Hwang, Yong Sup

AU - Cho, Myung Soo

AU - Moon, Shin Yong

AU - Kim, Dong Wook

AU - Chang, Jin Woo

N1 - Funding Information: This research was supported by a Grant (03-PJ1-PG1-CH07-0004) from the Korea Health 21 R&D Project, Ministry of Health & Welfare and Grants (codes: SC1020, SC2140, and SC2160) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are grateful to Dr. Kwang-Soo Kim at Harvard Medical School/Mclean Hospital for the gift of N2 cells.

PY - 2006/3/3

Y1 - 2006/3/3

N2 - To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

AB - To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

KW - Behavior recovery

KW - Differentiation

KW - Dopamine neurons

KW - ES cells

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=30944461146&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.12.140

DO - 10.1016/j.bbrc.2005.12.140

M3 - Article

C2 - 16412382

AN - SCOPUS:30944461146

SN - 0006-291X

VL - 341

SP - 6

EP - 12

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this