Abstract
To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.
| Original language | English |
|---|---|
| Pages (from-to) | 6-12 |
| Number of pages | 7 |
| Journal | Biochemical and biophysical research communications |
| Volume | 341 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Mar 3 |
| Externally published | Yes |
Bibliographical note
Funding Information:This research was supported by a Grant (03-PJ1-PG1-CH07-0004) from the Korea Health 21 R&D Project, Ministry of Health & Welfare and Grants (codes: SC1020, SC2140, and SC2160) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are grateful to Dr. Kwang-Soo Kim at Harvard Medical School/Mclean Hospital for the gift of N2 cells.
Keywords
- Behavior recovery
- Differentiation
- Dopamine neurons
- ES cells
- Parkinson's disease
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology