Doxapram increases corticotropin-releasing factor immunoreactivity and mRNA expression in the rat central nucleus of the amygdala

Song Hyen Choi; Sung Jin Kim; Sang Ha Park; Bo Hyun Moon; Eunju Do; Boe Gwun Chun; Min Soo Lee; Kyung Ho Shin

doi:10.1016/j.peptides.2005.03.036

Doxapram increases corticotropin-releasing factor immunoreactivity and mRNA expression in the rat central nucleus of the amygdala

Song Hyen Choi, Sung Jin Kim, Sang Ha Park, Bo Hyun Moon, Eunju Do, Boe Gwun Chun, Min Soo Lee, Kyung Ho Shin

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Doxapram causes panic anxiety in humans. To determine whether doxapram alters corticotropin-releasing factor (CRF) expression in the central nucleus of the amygdala (CeA), paraventricular nucleus of hypothalamus (PVN), or bed nucleus of the stria terminalis (BNST), we used immunohistochemistry to measure CRF peptide in these brain areas after doxapram injection. Doxapram injection significantly increased CRF-like immunoreactivity (CRF-IR) within the CeA, but not in the BNST or PVN, and this increase was significant 2 h after injection. In addition, doxapram significantly increased CRF mRNA expression within the CeA, and this was most prominent 30 min after injection. These results suggest that doxapram selectively increases CRF expression within the CeA, and that this is mediated by increased CRF gene transcription. This increase in CRF-IR within the CeA might explain the doxapram-induced anxiety reaction.

Original language	English
Pages (from-to)	2246-2251
Number of pages	6
Journal	Peptides
Volume	26
Issue number	11
DOIs	https://doi.org/10.1016/j.peptides.2005.03.036
Publication status	Published - 2005 Nov

Bibliographical note

Funding Information:
This research was supported by a grant from the Brain Research Center of the 21st Century Frontier Research Program, funded by the Korean Ministry of Science and Technology (Grant No. M103KV010007 03K2201 00720). We thank Dr. Younglim Lee (Indiana University, Indianapolis, IN, USA) for helpful suggestions and a review of the manuscript. We are grateful to professor Kelly Mayo for his kind gift of full-length rat CRF cDNA.

Keywords

Amygdala
Anxiety
Corticotropin-releasing factor
Doxapram

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/j.peptides.2005.03.036

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title = "Doxapram increases corticotropin-releasing factor immunoreactivity and mRNA expression in the rat central nucleus of the amygdala",

abstract = "Doxapram causes panic anxiety in humans. To determine whether doxapram alters corticotropin-releasing factor (CRF) expression in the central nucleus of the amygdala (CeA), paraventricular nucleus of hypothalamus (PVN), or bed nucleus of the stria terminalis (BNST), we used immunohistochemistry to measure CRF peptide in these brain areas after doxapram injection. Doxapram injection significantly increased CRF-like immunoreactivity (CRF-IR) within the CeA, but not in the BNST or PVN, and this increase was significant 2 h after injection. In addition, doxapram significantly increased CRF mRNA expression within the CeA, and this was most prominent 30 min after injection. These results suggest that doxapram selectively increases CRF expression within the CeA, and that this is mediated by increased CRF gene transcription. This increase in CRF-IR within the CeA might explain the doxapram-induced anxiety reaction.",

keywords = "Amygdala, Anxiety, Corticotropin-releasing factor, Doxapram",

author = "Choi, {Song Hyen} and Kim, {Sung Jin} and Park, {Sang Ha} and Moon, {Bo Hyun} and Eunju Do and Chun, {Boe Gwun} and Lee, {Min Soo} and Shin, {Kyung Ho}",

note = "Funding Information: This research was supported by a grant from the Brain Research Center of the 21st Century Frontier Research Program, funded by the Korean Ministry of Science and Technology (Grant No. M103KV010007 03K2201 00720). We thank Dr. Younglim Lee (Indiana University, Indianapolis, IN, USA) for helpful suggestions and a review of the manuscript. We are grateful to professor Kelly Mayo for his kind gift of full-length rat CRF cDNA.",

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language = "English",

volume = "26",

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T1 - Doxapram increases corticotropin-releasing factor immunoreactivity and mRNA expression in the rat central nucleus of the amygdala

AU - Choi, Song Hyen

AU - Kim, Sung Jin

AU - Park, Sang Ha

AU - Moon, Bo Hyun

AU - Do, Eunju

AU - Chun, Boe Gwun

AU - Lee, Min Soo

AU - Shin, Kyung Ho

N1 - Funding Information: This research was supported by a grant from the Brain Research Center of the 21st Century Frontier Research Program, funded by the Korean Ministry of Science and Technology (Grant No. M103KV010007 03K2201 00720). We thank Dr. Younglim Lee (Indiana University, Indianapolis, IN, USA) for helpful suggestions and a review of the manuscript. We are grateful to professor Kelly Mayo for his kind gift of full-length rat CRF cDNA.

PY - 2005/11

Y1 - 2005/11

N2 - Doxapram causes panic anxiety in humans. To determine whether doxapram alters corticotropin-releasing factor (CRF) expression in the central nucleus of the amygdala (CeA), paraventricular nucleus of hypothalamus (PVN), or bed nucleus of the stria terminalis (BNST), we used immunohistochemistry to measure CRF peptide in these brain areas after doxapram injection. Doxapram injection significantly increased CRF-like immunoreactivity (CRF-IR) within the CeA, but not in the BNST or PVN, and this increase was significant 2 h after injection. In addition, doxapram significantly increased CRF mRNA expression within the CeA, and this was most prominent 30 min after injection. These results suggest that doxapram selectively increases CRF expression within the CeA, and that this is mediated by increased CRF gene transcription. This increase in CRF-IR within the CeA might explain the doxapram-induced anxiety reaction.

AB - Doxapram causes panic anxiety in humans. To determine whether doxapram alters corticotropin-releasing factor (CRF) expression in the central nucleus of the amygdala (CeA), paraventricular nucleus of hypothalamus (PVN), or bed nucleus of the stria terminalis (BNST), we used immunohistochemistry to measure CRF peptide in these brain areas after doxapram injection. Doxapram injection significantly increased CRF-like immunoreactivity (CRF-IR) within the CeA, but not in the BNST or PVN, and this increase was significant 2 h after injection. In addition, doxapram significantly increased CRF mRNA expression within the CeA, and this was most prominent 30 min after injection. These results suggest that doxapram selectively increases CRF expression within the CeA, and that this is mediated by increased CRF gene transcription. This increase in CRF-IR within the CeA might explain the doxapram-induced anxiety reaction.

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Doxapram increases corticotropin-releasing factor immunoreactivity and mRNA expression in the rat central nucleus of the amygdala

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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