TY - GEN
T1 - Droplet squeezing for highly effective gene delivery into human t lymphocytes
AU - Joo, Byeongju
AU - Lee, Hasung
AU - Yun, Seung Gyu
AU - Chung, Aram
N1 - Funding Information:
This study was supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2018R1D1A1B07045538).
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - We present a novel droplet-based intracellular delivery method for highly effective internalization of external macromolecules into human T lymphocytes. First, we generated droplets that encapsulate single cells mixed with biomolecules, using a flow-focusing droplet generator. The droplets containing cells were then passed through a series of narrow constrictions, creating transient nanopores in the cell membrane. Consequently, external cargos in droplets can be transported directly into the cell cytosol throughout the nanopores' lifespan, achieving effective intracellular delivery. This process was able to achieve highly efficient and high-throughput delivery of different nanomaterials into human T lymphocytes, while maintaining high cell viability.
AB - We present a novel droplet-based intracellular delivery method for highly effective internalization of external macromolecules into human T lymphocytes. First, we generated droplets that encapsulate single cells mixed with biomolecules, using a flow-focusing droplet generator. The droplets containing cells were then passed through a series of narrow constrictions, creating transient nanopores in the cell membrane. Consequently, external cargos in droplets can be transported directly into the cell cytosol throughout the nanopores' lifespan, achieving effective intracellular delivery. This process was able to achieve highly efficient and high-throughput delivery of different nanomaterials into human T lymphocytes, while maintaining high cell viability.
KW - Droplet Microfluidics
KW - Droplet Squeezing
KW - Gene Delivery
KW - Intracellular Delivery
UR - http://www.scopus.com/inward/record.url?scp=85098279750&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098279750
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 100
EP - 101
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -