Dual-color imaging of sodium/calcium ion activities with twophoton fluorescent probes

Hyung Joong Kim; Ji Hee Han; Mi Kyung Kim; Chang Su Lim; Hwan Myung Kim; Bong Rae Cho

doi:10.1002/anie.201002907

Dual-color imaging of sodium/calcium ion activities with twophoton fluorescent probes

Hyung Joong Kim, Ji Hee Han, Mi Kyung Kim, Chang Su Lim, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

A two-photon probe (BCaM) shows 14fold enhancement in two-photon emission fluorescence in response to Ca²⁺ and shows high sensitivity and selectivity for near-membrane Ca²⁺ ions (see picture). Combined with the known Na⁺ twophoton probe ANa1, BCaM allows simultaneous dual-color imaging of Ca²⁺/Na⁺ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching. (Figure Presented)

Original language	English
Pages (from-to)	6786-6789
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	38
DOIs	https://doi.org/10.1002/anie.201002907
Publication status	Published - 2010 Sept 10

Keywords

Calcium
Fluorescence spectroscopy
Imaging agents
Live tissue
Two-photon microscopy

ASJC Scopus subject areas

Catalysis
General Chemistry

Access to Document

10.1002/anie.201002907

Cite this

@article{98561efca83a499aae564e1164b15c05,

title = "Dual-color imaging of sodium/calcium ion activities with twophoton fluorescent probes",

abstract = "A two-photon probe (BCaM) shows 14fold enhancement in two-photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near-membrane Ca2+ ions (see picture). Combined with the known Na+ twophoton probe ANa1, BCaM allows simultaneous dual-color imaging of Ca2+/Na+ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching. (Figure Presented)",

keywords = "Calcium, Fluorescence spectroscopy, Imaging agents, Live tissue, Two-photon microscopy",

author = "Kim, {Hyung Joong} and Han, {Ji Hee} and Kim, {Mi Kyung} and Lim, {Chang Su} and Kim, {Hwan Myung} and Cho, {Bong Rae}",

year = "2010",

month = sep,

day = "10",

doi = "10.1002/anie.201002907",

language = "English",

volume = "49",

pages = "6786--6789",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "38",

}

TY - JOUR

T1 - Dual-color imaging of sodium/calcium ion activities with twophoton fluorescent probes

AU - Kim, Hyung Joong

AU - Han, Ji Hee

AU - Kim, Mi Kyung

AU - Lim, Chang Su

AU - Kim, Hwan Myung

AU - Cho, Bong Rae

PY - 2010/9/10

Y1 - 2010/9/10

N2 - A two-photon probe (BCaM) shows 14fold enhancement in two-photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near-membrane Ca2+ ions (see picture). Combined with the known Na+ twophoton probe ANa1, BCaM allows simultaneous dual-color imaging of Ca2+/Na+ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching. (Figure Presented)

AB - A two-photon probe (BCaM) shows 14fold enhancement in two-photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near-membrane Ca2+ ions (see picture). Combined with the known Na+ twophoton probe ANa1, BCaM allows simultaneous dual-color imaging of Ca2+/Na+ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching. (Figure Presented)

KW - Calcium

KW - Fluorescence spectroscopy

KW - Imaging agents

KW - Live tissue

KW - Two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=77956591934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956591934&partnerID=8YFLogxK

U2 - 10.1002/anie.201002907

DO - 10.1002/anie.201002907

M3 - Article

C2 - 20715036

AN - SCOPUS:77956591934

SN - 1433-7851

VL - 49

SP - 6786

EP - 6789

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 38

ER -

Dual-color imaging of sodium/calcium ion activities with twophoton fluorescent probes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this