Dual phase-detected infrared photothermal microscopy

Chanjong Park, Minhaeng Cho

Research output: Contribution to journalArticlepeer-review


Infrared photothermal microscopy (IPM) has recently gained considerable attention as a versatile analytical platform capable of providing spatially resolved molecular insights across diverse research fields. This technique has led to numerous breakthroughs in the study of compositional variations in functional materials and cellular dynamics in living cells. However, its application to investigate multiple components of temporally dynamic systems, such as living cells and operational devices, has been hampered by the limited information content of the IP signal, which only covers a narrow spectral window (< 1 cm−1). Here, we present a straightforward approach for measuring two distinct IPM images utilizing the orthogonality between the in-phase and quadrature outputs of a lock-in amplifier, called dual-phase IR photothermal (DP-IP) detection. We demonstrate the feasibility of DP-IP detection for IPM in distinguishing two different micro-sized polymer beads.

Original languageEnglish
Pages (from-to)6865-6875
Number of pages11
JournalOptics Express
Issue number5
Publication statusPublished - 2024 Feb 26

Bibliographical note

Publisher Copyright:
© 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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