Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media

Vo Van Giau, Yoon Hee Park, Kyu Hwan Shim, Sang Wook Son, Seong Soo A. An

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomaterial’s surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography–mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)90-97
Number of pages8
JournalFrontiers of Chemical Science and Engineering
Issue number1
Publication statusPublished - 2019 Mar 1


  • ClueGO
  • nanoparticles
  • protein corona
  • ZnONPs

ASJC Scopus subject areas

  • General Chemical Engineering


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