DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

Paul D. Dunne, Ricardo A. Fernandes, James McColl, Won Yoon Ji, John R. James, Simon J. Davis, David Klenerman

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small.

Original languageEnglish
Pages (from-to)L5-L7
JournalBiophysical Journal
Volume97
Issue number4
DOIs
Publication statusPublished - 2009 Aug 19
Externally publishedYes

Bibliographical note

Funding Information:
This project was funded by the Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, Medical Research Council and The Wellcome Trust, UK, and by Fundação para a Ciência e a Tecnologia, Portugal.

ASJC Scopus subject areas

  • Biophysics

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